Brown I I, Fadeyev S I, Kirik I I, Severina I I, Skulachev V P
Department of Biology, Odessa State University, USSR.
FEBS Lett. 1990 Sep 17;270(1-2):203-6. doi: 10.1016/0014-5793(90)81268-s.
Light-dependent Na+ and H+ transports, membrane potential (delta psi) and motility have been studied in the cells of the marine cyanobacterium Oscillatoria brevis. In the presence of a protonophorous uncoupler, carbonyl cyanide-m-chlorophenylhydrazone, the intracellular Na+ level is shown to increase in the dark and decrease in the light. The Na+/H+ antiporter, monensin, stimulates the dark CCCP-dependent [Na+]in increase and abolishes the light-dependent [Na+]in decrease. Na+ ions are necessary for the fast light-induced delta psi generation and H+ uptake by the cells. This uptake is inhibited by monensin being resistant to CCCP. Monensin sensitizes the delta psi level and the motility rate to low CCCP concentrations. The obtained data are consistent with the assumption that O. brevis possesses a primary Na+ pump which utilizes (directly or indirectly) the light energy.
对海洋蓝藻短颤藻（Oscillatoria brevis）细胞中的光依赖性钠（Na⁺）和氢（H⁺）转运、膜电位（δψ）及运动性进行了研究。在存在质子载体解偶联剂羰基氰化物 - m - 氯苯腙（CCCP）的情况下，细胞内的Na⁺水平在黑暗中升高，在光照下降低。Na⁺/H⁺反向转运蛋白莫能菌素能刺激黑暗中依赖CCCP的细胞内[Na⁺]升高，并消除光照下依赖的细胞内[Na⁺]降低。Na⁺离子对于细胞快速光诱导的δψ产生和H⁺摄取是必需的。这种摄取受到莫能菌素的抑制，而对CCCP具有抗性。莫能菌素使δψ水平和运动速率对低浓度CCCP敏感。所获得的数据与短颤藻拥有一个直接或间接利用光能的初级Na⁺泵这一假设一致。
