Ackermann Maximilian, Wolloscheck Tanja, Wellmann Axel, Li Vincent W, Li William W, Konerding Moritz A
Institute of Functional and Clinical Anatomy, Johannes Gutenberg University Mainz, Mainz, Germany.
Eur Surg Res. 2011;47(2):81-9. doi: 10.1159/000328143. Epub 2011 Jun 30.
Numerous proangiogenic growth factors have been shown to improve impaired wound healing. This study evaluated the effects of subcutaneous pretreatment with a combination of proangiogenic growth factors on wound closure, mechanical properties, vessel density, and morphology.
Thirty-six Balb/c mice with streptozotocin-induced diabetes were divided into 3 groups. A mixture of VEGF (35.0 μg), bFGF (2.5 μg), and PDGF (3.5 μg) was administered subcutaneously 3, 5, and 7 days prior to wounding in the first group, whereas the second group received three doses of 3.5 μg PDGF. Wound sizes were assessed daily and the repaired tissues were harvested 7 days after wound closure.
Complete closure (≥95% healing of initial wound area) was reached in all proangiogenic pretreated animals by day 17, whereas the PDGF monotherapy group needed up to 20 days for complete closure. By the time of tissue harvesting on day 24, complete closure was not reached in all control animals. Punch biopsy material revealed 1.6-fold higher vessel densities in the proangiogenic combination-pretreated group than in the controls.
Proangiogenic priming revealed several significant effects on diabetic wound healing: faster time to closure, a higher vessel density, and improved functional outcome.
众多促血管生成生长因子已被证明可改善受损的伤口愈合。本研究评估了促血管生成生长因子联合皮下预处理对伤口闭合、力学性能、血管密度和形态的影响。
将36只链脲佐菌素诱导糖尿病的Balb/c小鼠分为3组。第一组在受伤前3天、5天和7天皮下注射VEGF（35.0μg）、bFGF（2.5μg）和PDGF（3.5μg）的混合物，而第二组接受三剂3.5μg的PDGF。每天评估伤口大小，并在伤口闭合后7天收获修复组织。
到第17天，所有接受促血管生成预处理的动物伤口完全闭合（初始伤口面积愈合≥95%），而PDGF单一疗法组完全闭合需要长达20天。在第24天组织收获时，所有对照动物未实现完全闭合。打孔活检材料显示，促血管生成联合预处理组的血管密度比对照组高1.6倍。
促血管生成预处理对糖尿病伤口愈合显示出若干显著影响：更快的闭合时间、更高的血管密度和更好的功能结果。
