Institute of Functional and Clinical Anatomy, University Medical Center of Johannes Gutenberg University Mainz, Mainz, Germany.
Int J Mol Med. 2011 May;27(5):647-53. doi: 10.3892/ijmm.2011.641. Epub 2011 Mar 3.
Growth factors and/or angiogenic factors are supposed to improve wound healing. The aim of our study was to evaluate the effects of subcutaneous pretreatment with combinatory proangiogenic factors on wound closure, mechanical properties, vessel density and morphology. Twenty-eight Balb/c mice were divided equally into two groups. A mixture of VEGF (35.0 µg), bFGF (2.5 µg) and PDGF (3.5 µg) was administered subcutaneously 3, 5 and 7 days to 14 mice before full thickness skin punch biopsy wounding, whereas 14 control animals received three injections of 0.2 ml saline solution. Wound sizes were assessed daily and the repaired tissues were harvested 7 days after complete wound closure. Complete closure (≥ 95% healing of initial wound area) was reached in all proangiogenic pretreated animals on day 10, whereas controls needed 13 days for complete closure. Tensile strengths were nearly twofold higher compared to the controls (p ≤ 0.01). The punch biopsy material revealed 4.2-fold higher vessel densities in the proangiogenic pretreated group. On day 17, the vessel densities in the proangiogenic pretreated wounds were also 3.2-fold higher compared to the untreated controls. No significant differences were seen in the collagen ratio. Pretreatment with proangiogenic factors revealed several significant effects on wound healing: faster time to closure, a higher vessel density and a better functional outcome. These results suggest a beneficial effect of pretreatment with combinatory growth factors in mouse skin wounds without impaired wound healing. This might be exploited in further investigations in diabetic healing as a therapeutic approach for elective surgery.
生长因子和/或血管生成因子被认为可以改善伤口愈合。我们的研究旨在评估皮下预处理联合促血管生成因子对伤口闭合、机械性能、血管密度和形态的影响。将 28 只 Balb/c 小鼠平均分为两组。一组 14 只小鼠在全层皮肤打孔活检性创伤前 3、5 和 7 天，皮下注射 VEGF（35.0μg）、bFGF（2.5μg）和 PDGF（3.5μg）混合物，而另一组 14 只对照动物接受 3 次 0.2ml 生理盐水注射。每天评估伤口大小，在完全闭合伤口后 7 天采集修复组织。所有促血管生成预处理动物均在第 10 天达到完全闭合（初始伤口面积的≥95%愈合），而对照动物则需要 13 天才能完全闭合。与对照组相比，拉伸强度几乎提高了两倍（p≤0.01）。促血管生成预处理组的血管密度比对照组高 4.2 倍。在第 17 天，促血管生成预处理伤口的血管密度也比未处理的对照组高 3.2 倍。胶原比没有显著差异。促血管生成因子预处理对伤口愈合有多种显著影响：闭合时间更快、血管密度更高、功能结果更好。这些结果表明，在不影响伤口愈合的情况下，联合生长因子预处理对小鼠皮肤伤口具有有益的效果。这可能会在糖尿病愈合的进一步研究中作为择期手术的治疗方法得到利用。
