Zabanov S A, Vasil'eva L A, Ratner V A
Genetika. 1990 Jul;26(7):1144-53.
A series of subpopulations earlier obtained were studied for hybridization of mobile genetic elements (MGE). The subpopulations examined were two "selected" (ris- and ris+), two "temperature" (ri(c113) and ri(c149)) and the control (ric). The method of in situ hybridization with polytene chromosomes of larval salivary glands was used to determine the patterns of MGE localization for all subpopulations. The patterns obtained appeared to be quite different from that of mdg-2. The trees of similarity for subpopulations according to the patterns of every MGE localization were built by conventional clustering methods. These trees were topologically similar to each other and to mdg-2. Distinction spectra of patterns of four daughter subpopulations, in comparison with the control one, were shown for each of these MGE to be independent and individual. However, there are some common regularities among copia-like MGE-mdg-1, copia, mdg-2 and, probably, mdg-3, namely: non-random property of the majority of changes, the similarity of patterns for subpopulations with similar phenotypes etc. So, Drosophila genome can be conceived as a complex system of patterns of different MGE localization, capable of common or independent mass transpositions after external stress action.
对先前获得的一系列亚群进行了移动遗传元件（MGE）杂交研究。所检测的亚群有两个“选择的”（ris-和ris+）、两个“温度相关的”（ri(c113)和ri(c149)）以及对照（ric）。采用幼虫唾液腺多线染色体原位杂交方法来确定所有亚群中MGE的定位模式。所获得的模式似乎与mdg-2的模式有很大不同。根据每个MGE的定位模式，通过传统聚类方法构建了亚群的相似性树。这些树在拓扑结构上彼此相似，并且与mdg-2相似。对于这些MGE中的每一个，与对照亚群相比，四个子代亚群的模式区分光谱显示是独立且独特的。然而，在类copia的MGE-mdg-1、copia、mdg-2以及可能的mdg-3之间存在一些共同规律，即：大多数变化的非随机性、具有相似表型的亚群模式的相似性等。因此，可以将果蝇基因组设想为一个由不同MGE定位模式组成的复杂系统，在外部应激作用后能够进行共同或独立的大规模转座。
