University of Southern California, Department of Biomedical Engineering, Los Angeles, California 90089, USA.
J Biomed Opt. 2011 Jun;16(6):066007. doi: 10.1117/1.3589299.
We report a novel in vivo spectral imaging approach to cancer detection and chemotherapy assessment. We describe and characterize a ratiometric spectral imaging and analysis method and evaluate its performance for tumor detection and delineation by quantitatively monitoring the specific accumulation of targeted gallium corrole (HerGa) into HER2-positive (HER2 +) breast tumors. HerGa temporal accumulation in nude mice bearing HER2 + breast tumors was monitored comparatively by a. this new ratiometric imaging and analysis method; b. established (reflectance and fluorescence) spectral imaging; c. more commonly used fluorescence intensity imaging. We also tested the feasibility of HerGa imaging in vivo using the ratiometric spectral imaging method for tumor detection and delineation. Our results show that the new method not only provides better quantitative information than typical spectral imaging, but also better specificity than standard fluorescence intensity imaging, thus allowing enhanced in vivo outlining of tumors and dynamic, quantitative monitoring of targeted chemotherapy agent accumulation into them.
我们报告了一种用于癌症检测和化疗评估的新型体内光谱成像方法。我们描述并表征了一种比率光谱成像和分析方法，并通过定量监测靶向镓卟啉（HerGa）特异性积累到 HER2 阳性（HER2+）乳腺癌肿瘤中来评估其对肿瘤检测和描绘的性能。通过以下方式比较监测裸鼠中 HER2+乳腺癌肿瘤中 HerGa 的时间积累：a. 这种新的比率成像和分析方法；b. 建立的（反射和荧光）光谱成像；c. 更常用的荧光强度成像。我们还测试了使用比率光谱成像方法进行肿瘤检测和描绘的 HerGa 体内成像的可行性。我们的结果表明，该新方法不仅提供了比典型光谱成像更好的定量信息，而且比标准荧光强度成像具有更好的特异性，从而允许更好地在体内勾勒出肿瘤，并对靶向化疗药物积累到肿瘤中进行动态、定量监测。
