Hwang Jae Youn, Gross Zeev, Gray Harry B, Medina-Kauwe Lali K, Farkas Daniel L
Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA ; Department of Biomedical Engineering, University of Southern California, Los Angeles, CA.
Beckman Research Institute, California Institute of Technology, Pasadena, CA ; Department of Chemistry, Technion-Israel Institute of Technology, Haifa.
Proc SPIE Int Soc Opt Eng. 2011 Feb 28;7902. doi: 10.1117/12.877780.
We report the feasibility of tumor detection and delineation using multimode optical imaging of targeted gallium corrole (HerGa). HerGa is highly effective for targeted HER2+ tumor elimination , and it emits intense fluorescence. These unique characteristics of HerGa prompted us to investigate the potential of HerGa for tumor detection and delineation, by performing multimode optical imaging and ; the imaging modes included fluorescence intensity, spectral (including ratiometric), lifetime, and two-photon excited fluorescence, using our custom-built imaging system. While fluorescence intensity imaging provided information about tumor targeting capacity and tumor retention of HerGa, ratiometric spectral imaging offered more quantitative and specific information about HerGa location and accumulation. Most importantly, the fluorescence lifetime imaging of HerGa allowed us to discriminate between tumor and non-tumor regions by fluorescence lifetime differences. Finally, two-photon excited fluorescence images provided highly resolved and thus topologically detailed information around the tumor regions where HerGa accumulates. Taken together, the results shown in this report suggest the feasibility of tumor detection and delineation by multimode optical imaging of HerGa, and fluorescent chemotherapy agents in general. Specifically, the multimode optical imaging can offer complementary and even synergetic information simultaneously in the tumor detection and delineation by HerGa, thus enhancing contrast.
我们报告了使用靶向镓卟啉（HerGa）的多模态光学成像进行肿瘤检测和勾勒的可行性。HerGa对靶向消除HER2+肿瘤非常有效，并且会发出强烈的荧光。HerGa的这些独特特性促使我们通过进行多模态光学成像来研究HerGa在肿瘤检测和勾勒方面的潜力；成像模式包括荧光强度、光谱（包括比率）、寿命和双光子激发荧光，使用我们定制的成像系统。虽然荧光强度成像提供了有关HerGa的肿瘤靶向能力和肿瘤滞留情况的信息，但比率光谱成像提供了有关HerGa位置和积累的更定量和具体的信息。最重要的是，HerGa的荧光寿命成像使我们能够通过荧光寿命差异区分肿瘤和非肿瘤区域。最后，双光子激发荧光图像提供了高分辨率的、因此在HerGa积累的肿瘤区域周围具有拓扑细节的信息。综上所述，本报告所示结果表明通过HerGa以及一般荧光化疗药物的多模态光学成像进行肿瘤检测和勾勒是可行的。具体而言，多模态光学成像可以在HerGa进行肿瘤检测和勾勒时同时提供互补甚至协同的信息，从而增强对比度。
