University of California, San Diego, Department of Chemistry and Biochemistry, 9500 Gilman Drive, La Jolla, California 92093, USA.
J Biomed Opt. 2011 Jun;16(6):066012. doi: 10.1117/1.3593003.
A simple method to fabricate Eu(3+) doped silica nanoshells particles with 100 and 200 nm diameters is reported. Amino polystyrene beads were used as templates, and an 8 to 10 nm thick silica gel coating was formed by the sol-gel reaction. After removing the template by calcination, porous dehydrated silica gel nanoshells of uniform size were obtained. The Eu(3+) doped silica nanoshells exhibited a red emission at 615 nm on UV excitation. The porous structure of the silica shell wall was characterized by transmission electron microscopy measurements, while particle size and zeta potentials of the particles suspended in aqueous solution were characterized by dynamic light scattering. Two-photon microscopy was used to image the nanoshells after assimilation by HeLa cancer cells.
本文报道了一种简单的方法来制备直径为 100nm 和 200nm 的掺 Eu(3+)的二氧化硅纳米壳粒子。采用氨基聚苯乙烯珠为模板，通过溶胶-凝胶反应形成 8 至 10nm 厚的二氧化硅凝胶涂层。通过煅烧除去模板后，得到尺寸均匀的多孔脱水二氧化硅纳米壳。在紫外光激发下，Eu(3+)掺杂的二氧化硅纳米壳在 615nm 处表现出红色发射。通过透射电子显微镜测量来表征二氧化硅壳壁的多孔结构，而通过动态光散射来表征悬浮在水溶液中的粒子的粒径和 ζ 电位。使用双光子显微镜来对 HeLa 癌细胞摄取后的纳米壳进行成像。
