与心血管危险因素相关的抗氧化酶活性的年龄相关性变化。

Age-related modification of antioxidant enzyme activities in relation to cardiovascular risk factors.

机构信息

Department of Nutritional Sciences, University of Vienna, Vienna, Austria.

出版信息

Eur J Clin Invest. 2012 Jan;42(1):42-8. doi: 10.1111/j.1365-2362.2011.02554.x. Epub 2011 Jul 4.

DOI:10.1111/j.1365-2362.2011.02554.x

PMID:21722101

Abstract

BACKGROUND

Since oxidative stress might cause and promote cardiovascular risk factors such as oxidized low-density lipoproteins (oxLDL), apolipoprotein(a) [apo(a)], asymmetric dimethylarginine (ADMA) and fetuin A, we investigated antioxidant enzyme activities in relation to the vascular redox balance and these risk factors in elderly people.

MATERIALS AND METHODS

For this observational study, a total of 102 subjects were recruited and divided into three groups: A (70-74 years/n = 48), B (75-79 years/n = 35) and C (≥ 80 years/n = 19). Activities of the erythrocyte antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) were determined photometrically oxLDL, apo(a), ADMA and fetuin A by ELISA. Plasma concentrations of the lipid peroxidation products malondialdehyde (MDA) and conjugated dienes (CD) were analysed with HPLC.

RESULTS

There were no significant age-associated alterations in apo(a) levels, but there was a significant age-related decrease in activities of SOD (A>C, B>C: P < 0·01), CAT (A>C: P < 0·05) and GSH-Px (A>C: P < 0·05), accompanied by a significant increase in oxLDL (A<C: P < 0·001; B<C: P < 0·05), ADMA (A<B: P < 0·05; A<C: P < 0·001), MDA (A<C, B<C: P < 0·01) and CD (A<C, B<C: P < 0·01), and a significant decrease in fetuin A (A>C: P < 0·01; B>C: P < 0·05). Consequently, all groups showed significant negative age-associated correlations between CAT and MDA (A, B, C: P < 0·05), GSH-Px and CD (A, C: P < 0·01; B: P < 0·05), SOD and oxLDL (A, B: P < 0·05; C: P < 0·01), and fetuin A and MDA (A: P < 0·01; B, C: P < 0·05), and a significant positive correlation between oxLDL and ADMA (A, B: P < 0·05; C: P < 0·01).

CONCLUSIONS

This study indicates a significant age-related decrease in antioxidant enzyme activities accompanied by significantly increased systemic oxidative stress, which promotes the cardiovascular risk factors oxLDL, ADMA and fetuin A in elderly people.

摘要

背景

由于氧化应激可能导致并促进心血管危险因素的形成和发展，如氧化型低密度脂蛋白（oxLDL）、载脂蛋白（a）[apo（a）]、不对称二甲基精氨酸（ADMA）和胎球蛋白 A，我们研究了抗氧化酶活性与血管氧化还原平衡以及老年人这些危险因素之间的关系。

材料和方法

在这项观察性研究中，共招募了 102 名受试者，并将其分为三组：A 组（70-74 岁/n=48）、B 组（75-79 岁/n=35）和 C 组（≥80 岁/n=19）。通过酶联免疫吸附试验（ELISA）测定红细胞抗氧化酶超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的活性。采用高效液相色谱法（HPLC）分析脂质过氧化产物丙二醛（MDA）和共轭二烯（CD）的血浆浓度。

结果

apo（a）水平与年龄无显著相关性，但 SOD（A>C，B>C：P<0·01）、CAT（A>C：P<0·05）和 GSH-Px（A>C：P<0·05）的活性随年龄显著降低，同时 oxLDL（A<C：P<0·001；B<C：P<0·05）、ADMA（A<B：P<0·05；A<C：P<0·001）、MDA（A<C，B<C：P<0·01）和 CD（A<C，B<C：P<0·01）显著增加，胎球蛋白 A 显著降低（A>C：P<0·01；B>C：P<0·05）。因此，所有组的 CAT 与 MDA（A、B、C：P<0·05）、GSH-Px 与 CD（A、C：P<0·01；B：P<0·05）、SOD 与 oxLDL（A、B：P<0·05；C：P<0·01）和胎球蛋白 A 与 MDA（A：P<0·01；B、C：P<0·05）之间均呈显著负相关，oxLDL 与 ADMA（A、B：P<0·05；C：P<0·01）之间呈显著正相关。