巴西里约热内卢产超广谱β-内酰胺酶大肠埃希菌的分子特征。
Molecular characteristics of extended-spectrum β-lactamase-producing Escherichia coli from Rio de Janeiro, Brazil.
机构信息
Division of Microbiology, Calgary Laboratory Services, Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, AB, Canada.
出版信息
Clin Microbiol Infect. 2011 Jul;17(7):1039-43. doi: 10.1111/j.1469-0691.2010.03440.x. Epub 2011 Jan 28.
Twenty-five extended-spectrum β-lactamase (ESBL)-producing Escherichia coli clinical isolates from Rio de Janeiro, Brazil were characterized by isoelectric focusing, PCR and sequencing of bla(ESBL) genes, plasmid-mediated quinolone resistance determinants, phylogenetic groups, replicon typing, pulsed-field electrophoresis, and multilocus sequencing typing. Twenty-three (92%) ESBL-producing E. coli isolates were positive for bla(CTX-M) genes, aac(6')-Ib-cr, and qnrB. Genetic relatedness of ESBL producers clustered seven (28%) CTX-M-15-producing isolates as sequence type (ST)410, clonal complex (CC) 23, and two (8%) as clone O25-ST131. Our results illustrate the predominance of phylogroup A (52%), ST410 (CC 23) and CTX-M-15 among ESBL-producing E. coli isolates from hospitals in Rio de Janeiro.
25 株产超广谱β-内酰胺酶（ESBL）的大肠埃希菌临床分离株来自巴西里约热内卢，通过等电聚焦、PCR 和 bla(ESBL)基因、质粒介导的喹诺酮耐药决定因素、系统发育群、复制子分型、脉冲场电泳和多位点序列分型进行了表征。23 株（92%）产 ESBL 的大肠埃希菌分离株bla(CTX-M)基因、aac(6')-Ib-cr 和 qnrB 阳性。ESBL 产生菌的遗传相关性将 7 株（28%）CTX-M-15 产生菌聚类为 ST410（CC23）和 2 株（8%）克隆 O25-ST131。我们的结果表明，里约热内卢医院产 ESBL 的大肠埃希菌分离株中，以 A 群（52%）、ST410（CC23）和 CTX-M-15 为主。
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