Oliveira Elisangela de, Oshiro Elisa Teruya, Pinto Rebeca Vieira, Castro Bruna Corrêa de, Daniel Karla Borges, Oliveira Janaina Michelle de, Júnior Manoel Sebastião da Costa Lima, Guimarães Euripedes Batista, Silva Jesiel Mamedes, Dorval Maria Elizabeth Cavalheiros
Laboratório de Parasitologia, Departamento de Patologia,Centro de Ciências Biológicas e da Saúde, Faculdade de Medicina, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS, Brazil.
Rev Bras Parasitol Vet. 2011 Apr-Jun;20(2):97-102. doi: 10.1590/s1984-29612011000200002.
Visceral leishmaniasis (VL) is a severe chronic disease caused by Leishmania (Leishmania) infantum chagasi. Better knowledge on the effects caused by this disease can help develop adequate clinical management and treatment. Parasitological and immunohistochemical studies were performed golden hamsters Mesocricetus auratus infected with bone marrow from individuals with VL in the State of Mato Grosso do Sul, central-west Brazil. The effects of parasitism in the spleen, liver, kidneys, lungs, heart and brain of the animals were examined. Eighteen hamsters were inoculated intraperitoneally, and six healthy animals were used as negative controls. The animals were kept in the animal house and checked for clinical signs. Specimens of each organ were examined for the presence of amastigotes. Immunohistochemical technique was performed in all brain specimens and organs negative on the direct examination of parasites. Direct examination of amastigotes was positive in the spleen and liver of all infected animals; 33.3% showed the parasite in the kidneys and lungs, and 16.7% in the heart. Parasitic forms were seen in 83.3% (15/18) of the brain examined. Immunohistochemistry confirmed the results of the direct examination, except in two specimens of lung tissue and in the brain specimens. Other studies are needed to further clarify the effect of the parasite in the central nervous system.
内脏利什曼病（VL）是由婴儿利什曼原虫（Leishmania）恰加斯亚种引起的一种严重慢性疾病。深入了解该疾病所造成的影响有助于制定恰当的临床管理和治疗方案。对来自巴西中西部南马托格罗索州患有内脏利什曼病个体的骨髓感染金黄仓鼠（Mesocricetus auratus）进行了寄生虫学和免疫组织化学研究。检查了寄生虫感染对动物脾脏、肝脏、肾脏、肺、心脏和大脑的影响。18只仓鼠经腹腔接种，6只健康动物用作阴性对照。动物饲养在动物房并检查临床体征。检查每个器官的标本是否存在无鞭毛体。对所有大脑标本以及寄生虫直接检查呈阴性的器官进行免疫组织化学技术检测。所有感染动物的脾脏和肝脏中无鞭毛体直接检查呈阳性；33.3%的动物肾脏和肺中发现寄生虫，16.7%的动物心脏中发现寄生虫。在83.3%（15/18）的检查大脑中发现了寄生形式。免疫组织化学证实了直接检查的结果，但肺组织的两个标本和大脑标本除外。需要进一步开展其他研究以进一步阐明寄生虫在中枢神经系统中的作用。
