Júnior Aírton M C, de Amorim Carvalho Fernando A, de Oliveira Dantas Weslany, Gomes Luana C L, da Silva Andrezza B S, de Sousa Cavalcante Maria M A, de Oliveira Ingrid M, de Deus Moura de Lima Marina, Rizzo Márcia Dos Santos, de Carvalho Leite Carla Maria, Moura Selma Maria Dos Santos, de Deus Moura Lúcia de Fátima Almeida, da Silva Benedito B
Department of Morphology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil
Department of Biochemistry and Pharmacology, Federal University of Piauí, Piauí, Teresina 64049550, Brasil.
Exp Biol Med (Maywood). 2016 Feb;241(4):359-66. doi: 10.1177/1535370215614658. Epub 2015 Nov 13.
Leishmaniasis is considered a serious public health problem in several regions in Brazil and worldwide. This research aimed to perform a histopathological and proteomic study of parotid, submandibular and sublingual glands of BALB/c mice infected by Leishmania (L) infantum chagasi using histological, immunohistochemical and epifluorescence techniques. Twelve isogenic BALB/c male mice, around six- to eight-weeks old, were separated into two groups: the animals of the control group were injected with 0.15 ml of NaCl, while those in the experimental group were inoculated with 5 × 10(6) amastigote forms of Leishmania (L) infantum chagasi by the ip route. After 50 days, animals were euthanized and major salivary glands were collected to perform histological, immunohistochemical and epifluorescence techniques using anti-Caspase-2, anti-Ki-67 and anti-β-catenin antibodies, respectively. The histological and morphometric evaluation showed clusters of mononuclear inflammatory cells and a higher area and perimeter of the parotid gland. However, none of the salivary glands had morphophysiological impairment. There was no immunoreactivity to the anti-caspase-2 antibody and Ki67 expression in acinar and ductal cells in both groups. According to the immunofluorescence staining, the β-catenin antibodies did not show nuclear expression, suggesting no uncontrolled proliferation. The data obtained in this study showed population and morphological stability of major salivary glands after 50 days post-infection by Leishmania (L) infantum chagasi.
利什曼病在巴西的几个地区以及全球范围内都被视为严重的公共卫生问题。本研究旨在利用组织学、免疫组织化学和落射荧光技术，对感染恰加斯婴儿利什曼原虫（Leishmania (L) infantum chagasi）的BALB/c小鼠的腮腺、下颌下腺和舌下腺进行组织病理学和蛋白质组学研究。将12只6至8周龄的同基因BALB/c雄性小鼠分为两组：对照组动物注射0.15毫升氯化钠，而实验组动物通过腹腔注射途径接种5×10(6)个恰加斯婴儿利什曼原虫的无鞭毛体形式。50天后，对动物实施安乐死并收集主要唾液腺，分别使用抗半胱天冬酶-2、抗Ki-67和抗β-连环蛋白抗体进行组织学、免疫组织化学和落射荧光技术检测。组织学和形态计量学评估显示单核炎性细胞簇以及腮腺的面积和周长更大。然而，所有唾液腺均未出现形态生理损伤。两组的腺泡和导管细胞中抗半胱天冬酶-2抗体均无免疫反应性且Ki67表达阴性。根据免疫荧光染色，β-连环蛋白抗体未显示核表达，表明不存在不受控制的增殖。本研究获得的数据显示，在感染恰加斯婴儿利什曼原虫50天后，主要唾液腺的细胞数量和形态保持稳定。
