Telenti A, Marshall W F, Smith T F
Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota 55905.
J Clin Microbiol. 1990 Oct;28(10):2187-90. doi: 10.1128/jcm.28.10.2187-2190.1990.
The polymerase chain reaction (PCR) was used to study DNA extracted from the blood of 25 transplant patients, 5 patients with infectious mononucleosis, and 13 healthy subjects and autopsy or biopsy tissue from 29 patients with lymphoproliferative disorders. Primers were directed to conserved regions of the Epstein-Barr virus (EBV) genome encoding capsid protein gp220 and Epstein-Barr nuclear antigen 1. Specific EBV amplification was found in the blood of 11 of 25 transplant patients and all patients with infectious mononucleosis. All patients with lymphoproliferative disorders occurring in the presence of immunosuppression (eight organ transplant patients and two patients with acquired immunodeficiency syndrome) had biopsies positive for EBV by PCR. Only 1 of 19 samples from lymphomas or leukemias unrelated to immunosuppression contained EBV. PCR confirmed the very close association of EBV and lymphoproliferative disorders occurring in the presence of immunosuppression. The significance of detecting EBV sequences in the blood of transplant patients, particularly in relationship to lymphoproliferation, requires further study.
采用聚合酶链反应（PCR）对25例移植患者、5例传染性单核细胞增多症患者及13名健康受试者的血液，以及29例淋巴增殖性疾病患者的尸检或活检组织中的DNA进行研究。引物针对编码衣壳蛋白gp220和EB病毒核抗原1的EB病毒（EBV）基因组保守区域。在25例移植患者中的11例以及所有传染性单核细胞增多症患者的血液中发现了特异性EBV扩增。所有在免疫抑制状态下发生淋巴增殖性疾病的患者（8例器官移植患者和2例获得性免疫缺陷综合征患者）的活检组织经PCR检测EBV呈阳性。与免疫抑制无关的淋巴瘤或白血病的19份样本中只有1份含有EBV。PCR证实了EBV与免疫抑制状态下发生的淋巴增殖性疾病密切相关。检测移植患者血液中EBV序列的意义，尤其是与淋巴增殖的关系，有待进一步研究。
