Lujan Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, New Mexico, USA.
Biophys J. 2011 Jul 6;101(1):128-33. doi: 10.1016/j.bpj.2011.05.054.
Temperature-sensitive hydrogel polymers are utilized as responsive layers in various applications. Although the polymer's native characteristics have been studied extensively, details concerning its properties during interaction with biorelated structures are lacking. This work investigates the interaction between a thermoresponsive polymer cushion and different lipid membrane capping layers probed by neutron reflectometry. N-isopropylacrylamide copolymerized with methacroylbenzophenone first supported a lipid bilayer composed of 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (DPPE) and subsequently 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The polymer-membrane systems were investigated above and below the polymer transition temperature (37 and 25°C). Although the same cushion supported each lipid membrane, the polymer hydration profile and thickness were markedly different for DPPE and DPPC systems. Because DPPE and DPPC have different bending rigidities, these results establish that the polymer-membrane interaction is critically mediated by the mechanics of the membrane, providing better insight into cell-hydrogel interactions.
温度敏感水凝胶聚合物被用作各种应用中的响应层。尽管已经对聚合物的固有特性进行了广泛研究，但关于其与生物相关结构相互作用时的特性的细节却知之甚少。本工作通过中子反射测量研究了热响应聚合物垫与不同脂质膜覆盖层之间的相互作用。首先，与甲丙烯酸苯甲酮共聚的 N-异丙基丙烯酰胺支撑了由 1,2-二棕榈酰基-sn-甘油-3-磷酸乙醇胺（DPPE）和 1,2-二棕榈酰基-sn-甘油-3-磷酸胆碱（DPPC）组成的脂质双层。在聚合物转变温度（37 和 25°C）以上和以下研究了聚合物-膜系统。尽管相同的垫子支撑每个脂质膜，但 DPPE 和 DPPC 系统的聚合物水合程度和厚度明显不同。由于 DPPE 和 DPPC 的弯曲刚度不同，这些结果表明聚合物-膜相互作用受到膜力学的严格调节，从而更深入地了解了细胞-水凝胶相互作用。
