Ward R L, Sander D S, Schiff G M, Bernstein D I
Division of Clinical Virology, James N. Gamble Institute of Medical Research, Cincinnati, Ohio 45219.
J Infect Dis. 1990 Dec;162(6):1298-303. doi: 10.1093/infdis/162.6.1298.
In an evaluation of WC3 bovine rotavirus (serotype 6) vaccine in infants, some subjects experienced a natural serotype 1 rotavirus infection before vaccination and others after. Therefore, the effects of both WC3 and natural rotavirus strains as either primary or boosting immunogens on serotype-specific neutralizing antibody responses could be determined. After primary natural infection (symptomatic or asymptomatic), neutralizing antibody titers were highest to serotype 1 but were consistently high to serotype 3, and low titers (greater than or equal to 20) to serotypes 2 and 4 were often detected. Previous vaccination with WC3 had little effect on the magnitude of these responses. In contrast, subjects infected with serotype 1 strains before vaccination experienced large (average, 12-fold) rises in neutralizing antibody to human serotypes 1-4 when vaccinated with WC3. Thus, although WC3 and the natural strains are distinct serotypes, their epitopes were sufficiently similar that reinfection with WC3 could boost neutralizing antibody titers to human serotypes in subjects primed by a previous natural infection.
在一项针对婴儿的WC3牛轮状病毒（血清型6）疫苗评估中，一些受试者在接种疫苗前感染了自然血清型1轮状病毒，另一些则在接种后感染。因此，可以确定WC3和自然轮状病毒株作为主要或加强免疫原对血清型特异性中和抗体反应的影响。在初次自然感染（有症状或无症状）后，血清型1的中和抗体滴度最高，但血清型3的滴度一直很高，并且经常检测到血清型2和4的低滴度（大于或等于20）。先前接种WC3对这些反应的强度影响很小。相比之下，在接种疫苗前感染血清型1毒株的受试者在接种WC3后，对人血清型1-4的中和抗体大幅上升（平均12倍）。因此，尽管WC3和自然毒株是不同的血清型，但它们的表位足够相似，以至于在先前自然感染引发免疫的受试者中，再次感染WC3可提高对人血清型的中和抗体滴度。
