Clark H F, Borian F E, Modesto K, Plotkin S A
Wistar Institute of Anatomy and Biology, Philadelphia, PA 19104.
Vaccine. 1990 Aug;8(4):327-32. doi: 10.1016/0264-410x(90)90089-5.
A reassortant rotavirus, strain W179-9, was constructed bearing gene 9 of serotype 1 rotavirus strain WI79 and all other genes derived from bovine (serotype 6) rotavirus strain WC3. The antigenic phenotype of WI79-9 is bivalent: serotype 1 and serotype 6. WI79-9 administered orally at a dose of 10(7.5) p.f.u. induced no adverse effects in 48 infants of age 2-11 months. Serotype 1- and serotype 6-specific serum neutralizing antibody titres were induced with approximately equal frequency in these infants. Serotype 1-specific antibody responses were inhibited in infants previously seropositive to type 1. The immune response rate was enhanced by administration of a second, 'booster dose'.
构建了一种重配轮状病毒W179 - 9株，它携带1型轮状病毒WI79株的基因9，其他所有基因均来源于牛（6型）轮状病毒WC3株。WI79 - 9的抗原表型是双价的：1型和6型。以10(7.5)噬斑形成单位的剂量口服WI79 - 9，对48名2至11个月大的婴儿未产生不良反应。在这些婴儿中，1型和6型特异性血清中和抗体滴度的诱导频率大致相等。在先前对1型血清反应呈阳性的婴儿中，1型特异性抗体反应受到抑制。通过给予第二剂“加强剂量”，免疫反应率得到提高。
