Bioelectronic Systems Lab, Department of Electrical Engineering, Columbia University, 500 West 120th Street 1300, New York, NY, USA.
J Neural Eng. 2011 Aug;8(4):044003. doi: 10.1088/1741-2560/8/4/044003. Epub 2011 Jul 4.
We introduce a method to electrically stimulate individual neurons at single-cell resolution in arbitrary spatiotemporal patterns with precise control over stimulation thresholds. By exploiting a custom microelectronic chip, up to 65,000 non-Faradaic electrodes can be uniquely addressed with electrode density exceeding 6500 electrodes mm(-2). We demonstrate extracellular stimulation of dispersed primary hippocampal neuronal cultures using the chip at single-cell resolution.
我们介绍了一种方法，可以在任意时空模式下以单细胞分辨率对个体神经元进行电刺激，并对刺激阈值进行精确控制。通过利用定制的微电子芯片，可以用超过 6500 个电极 mm(-2)的电极密度对多达 65000 个非法拉第电极进行独特寻址。我们使用该芯片在单细胞分辨率下对分散的原代海马神经元培养物进行了细胞外刺激。
