Centre for High-Throughput Biology, University of British Columbia, Vancouver, British Columbia, Canada.
Nat Methods. 2011 Jul 3;8(8):649-51. doi: 10.1038/nmeth.1640.
We present a microfluidic 'megapixel' digital PCR device that uses surface tension-based sample partitioning and dehydration control to enable high-fidelity single DNA molecule amplification in 1,000,000 reactors of picoliter volume with densities up to 440,000 reactors cm(-2). This device achieves a dynamic range of 10(7), single-nucleotide-variant detection below one copy per 100,000 wild-type sequences and the discrimination of a 1% difference in chromosome copy number.
我们提出了一种微流控“百万像素”数字 PCR 设备，该设备使用基于表面张力的样品分区和脱水控制，可在皮升级别的 100 万个反应器中实现高达 44 万个/平方厘米的超高密度下，对单个 DNA 分子进行高保真扩增。该设备的动态范围为 10^7，单核苷酸变异检测下限为每 10 万个野生型序列中低于 1 个拷贝，并且能够区分 1%的染色体拷贝数差异。
