Department of Pathophysiology, Norman Bethune Medical School, Jilin University, Changchun 130021, PR China.
Oncol Rep. 2011 Oct;26(4):893-9. doi: 10.3892/or.2011.1376. Epub 2011 Jul 1.
T-cell factor 3 (TCF3), a downstream effector of Wnt signaling in embryonic stem (ES) cells, plays an important role in pluripotent self-renewal and proliferation. Loss of TCF3 delays the differentiation of mouse ES cells. The purpose of this study was to investigate the effect of TCF3 on embryonal carcinoma (EC). The mouse F9 EC cell line and a tumor-bearing mouse model were used to evaluate the anti-EC tumor effects of TCF3 in vitro and in vivo, respectively. The overexpression of TCF3 significantly inhibited proliferation, colony-forming and migration in F9 EC cells by approximately 30, 45 and 30%, respectively. The in vivo mouse model showed that the overexpression of TCF3 significantly reduced tumor volume (36.4%) and tumor weight (34.8%), malignancy progression and local infiltration and prolonged the life span of tumor-bearing mice. Overexpression of TCF3 significantly down-regulated Oct4 expression in the F9 EC cells. The results indicate that TCF3 is an inhibitor of the malignant phenotypes of embryonal carcinoma through the regulation of Oct4 expression.
T 细胞因子 3（TCF3）是胚胎干细胞（ES 细胞）中 Wnt 信号的下游效应因子，在多能性自我更新和增殖中发挥重要作用。TCF3 的缺失会延迟小鼠 ES 细胞的分化。本研究旨在探讨 TCF3 对胚胎癌细胞（EC）的影响。利用小鼠 F9 EC 细胞系和荷瘤小鼠模型，分别在体外和体内评估 TCF3 对 EC 的抗肿瘤作用。结果显示，TCF3 的过表达可使 F9 EC 细胞的增殖、集落形成和迁移分别减少约 30%、45%和 30%。体内小鼠模型表明，TCF3 的过表达可显著降低肿瘤体积（36.4%）和肿瘤重量（34.8%）、恶性进展和局部浸润，并延长荷瘤小鼠的生存期。TCF3 的过表达可显著下调 F9 EC 细胞中 Oct4 的表达。这些结果表明，TCF3 通过调节 Oct4 的表达，抑制胚胎癌细胞的恶性表型。
