Department of Pharmaceutical Engineering, Sangji University, Wonju 220-702, Korea.
Arch Pharm Res. 2011 Jun;34(6):971-8. doi: 10.1007/s12272-011-0614-1. Epub 2011 Jul 2.
To identify an analgesic/anti-inflammatory component from the leaves of Rhododendron aureum (Ericaceae), phytochemical isolation and pharmacological assays (writhing assays and vascular permeability assay for analgesic action in mice; carrageenan-induced paw edemaand TPA-induced ear edema assays of anti-inflammatory action in rats) were performed. Four compounds were isolated from the active fraction (BuOH fraction) by silica gel column chromatography and identified as (-)-rhododendrol, (-)-rhododendrin, avicularin and hyperoside by spectroscopic methods. Rhododendrin, the main compound of the BuOH fraction, exhibited significant analgesic actions in mice and anti-inflammatory actions in rats. This compound accounted for 3.1% of the MeOH extract and 0.48% of dried leaves, respectively, on HPLC analysis. These results suggest that rhododendrin is the major biologically active substance in the leaves of R. aureum with analgesic/anti-inflammatory activity.
为了从金顶杜鹃（杜鹃花科）的叶子中鉴定出一种具有镇痛/抗炎作用的成分，进行了植物化学分离和药理学检测（在小鼠中进行扭体实验和血管通透性实验以检测镇痛作用；在大鼠中进行角叉菜胶诱导的足肿胀和 TPA 诱导的耳肿胀实验以检测抗炎作用）。从活性部位（正丁醇部分）通过硅胶柱色谱法分离出四种化合物，通过光谱方法鉴定为（-）-杜鹃醇、（-）-杜鹃素、圣草酚和金丝桃苷。正丁醇部分的主要化合物杜鹃素在小鼠中表现出显著的镇痛作用，在大鼠中表现出抗炎作用。该化合物在 HPLC 分析中分别占甲醇提取物的 3.1%和干叶的 0.48%。这些结果表明，杜鹃素是具有镇痛/抗炎活性的金顶杜鹃叶子中的主要生物活性物质。
