Department of Cell Biology, Poznan University of Medical Sciences, Rokietnicka 5D, 60-806, Poznan, Poland.
Hum Cell. 2013 Mar;26(1):28-34. doi: 10.1007/s13577-011-0024-1. Epub 2011 Jul 3.
Phosphatidylserine membrane translocation (PST) is considered to be a marker of apoptosis; however, numerous studies have reported on its role in processes not related to cell death. The purpose of the study was to investigate: (1) what is the impact of PST on the motility of spermatozoa, and (2) does the swim-up isolation involve the percentage of cells presenting PST? Semen of 28 normozoospermic men (WHO criteria) was analyzed. High motility spermatozoa were isolated by the swim-up technique. The percentage of spermatozoa with PST in neat semen and after swim-up isolation was assessed with Annexin-V labeled with fluorescein, using flow cytometry technique. The spermatozoas' motility was measured with a computer-assisted analysis system. The kinetic subpopulations of spermatozoa were identified with dedicated software and analyzed regarding PST. Vital spermatozoa with PST demonstrated progressive movement. The motion analysis system revealed a very strong positive correlation between the percentage of vital spermatozoa with PST and the percentage of spermatozoa belonging to the slow subpopulation (r = 0.83; p < 0.05), as well as a very strong negative correlation between the percentage of vital spermatozoa with PST and the percentage of spermatozoa belonging to the rapid subpopulation (r = -0.86; p < 0.05). After the swim-up isolation, the percentage of vital spermatozoa presenting PST significantly decreased (2.4 ± 2.1% vs. 5.2 ± 2.4%; p < 0.05). Spermatozoa with PST present progressive movement; however, their motility is decreased. Isolation of spermatozoa with the swim-up technique eliminates the cells with PST.
磷脂酰丝氨酸膜易位（PST）被认为是细胞凋亡的标志；然而，许多研究已经报道了它在与细胞死亡无关的过程中的作用。本研究旨在探讨：（1）PST 对精子运动性的影响，以及（2）泳动分离是否涉及呈现 PST 的细胞百分比。分析了 28 名正常生育力男性（WHO 标准）的精液。通过泳动技术分离高活力精子。使用 Annexin-V 标记的荧光，用流式细胞术技术评估新鲜精液和泳动分离后精子的 PST 百分比。使用计算机辅助分析系统测量精子的运动性。使用专用软件识别精子的动力学亚群，并分析 PST。具有 PST 的有活力精子表现出进行性运动。运动分析系统显示，具有 PST 的有活力精子的百分比与属于慢速亚群的精子百分比之间存在很强的正相关（r = 0.83；p <0.05），与属于快速亚群的精子百分比之间存在很强的负相关（r = -0.86；p <0.05）。泳动分离后，具有 PST 的有活力精子的百分比显著降低（2.4 ± 2.1% vs. 5.2 ± 2.4%；p <0.05）。具有 PST 的精子表现出进行性运动；然而，它们的运动性降低。泳动技术分离精子可以消除具有 PST 的细胞。
