Ydiaquez-Miranda Mariela Adriana, Herrera-Barragán José Antonio, González-Lozano Miguel, Ávalos-Rodríguez Alejandro
Doctorate in Biological and Health Sciences, Metropolitan Autonomous University, Mexico City, Mexico.
Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, Mexico City, Mexico; .
Vet Res Forum. 2021;12(3):267-272. doi: 10.30466/vrf.2020.108342.2570. Epub 2021 Sep 15.
The aim of this study was to determine the potential fertilizing of spermatozoa from the epididymal tail in different periods of time post-orchiectomy (P-OQ). Therefore, the study was approached in two stages. In the first stage, the orchiectomy was performed in 30 adult pigs. The testicles were stored at 5.00 ˚C in physiological saline solution for 5, 24, 48, 72, 96 and 120 hr. The spermatozoa were obtained by backflushing the vas deferens. The spermogram and fluorometric study were performed for each sample to evaluate the exposure of phosphatidyl-serine (PS) and acrosome reaction (AR). The second stage included the fertilization test, 16 prepubertal sows were selected, after synchronizing the oestrous cycle and the post-cervical artificial insemination was performed with the refrigerated sperm samples from each P-OQ time. The percentage of live sperm remained without significant changes until 96 hr P-OQ. An increase in the percentage of spermatozoa that showed a PS exposure was observed. The premature AR was evident after 72 hr. Considering that the artificial insemination was performed ensuring a minimum number of live sperms, no significant differences were observed in the number of embryos and corpora lutea. The results indicated that pig sperm collected from the epididymal tail P-OQ and stored for 5 and up to 72 hr at 5.00 ˚C had viable characteristics and maintained their fertilization ability. However, there was an increase in the loss of phospholipid asymmetry of the plasma membrane as time increased (72 and 96 hr), therefore, sperm viability was decreased.
本研究的目的是确定在睾丸切除术后不同时间段（P - OQ）附睾尾部精子的潜在受精能力。因此，本研究分两个阶段进行。在第一阶段，对30头成年猪实施睾丸切除术。将睾丸在5.00˚C的生理盐水中保存5、24、48、72、96和120小时。通过输精管回冲获取精子。对每个样本进行精子分析和荧光测定研究，以评估磷脂酰丝氨酸（PS）的暴露情况和顶体反应（AR）。第二阶段包括受精试验，选择16头青春期前母猪，在同步发情周期后，用来自每个P - OQ时间的冷冻精子样本进行子宫颈后人工授精。直到P - OQ 96小时，活精子百分比没有显著变化。观察到显示PS暴露的精子百分比增加。72小时后明显出现过早顶体反应。考虑到人工授精时确保了最低数量的活精子，在胚胎和黄体数量上未观察到显著差异。结果表明，从附睾尾部P - OQ采集并在5.00˚C下保存5小时至72小时的猪精子具有存活特性并保持其受精能力。然而，随着时间增加（72小时和96小时），质膜磷脂不对称性的损失增加，因此精子活力下降。
