School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
J Zhejiang Univ Sci B. 2011 Jul;12(7):563-7. doi: 10.1631/jzus.B1100006.
In this paper, crude monkshood polysaccharide was isolated from Radix Aconiti Lateralis Preparata. The effects of crude monkshood polysaccharide on Escherichia coli and Staphylococcus aureus were investigated by microcalorimetry. The power-time curves of the bacterial growth at various concentrations (c) of crude monkshood polysaccharide were plotted with a TAM air isothermal microcalorimeter at 37 °C. The growth rate constant (μ), inhibitory ratio (I), peak-height (P(m)), and peak-time (t(m)) were calculated. From the data, the relationship between μ and c also was established. The growth rate constant μ decreased with the increasing concentrations of crude monkshood polysaccharide. Moreover, P(m) reduced and t(m) increased with increasing concentrations. The experimental results revealed that crude monkshood polysaccharide had inhibitory activity towards S. aureus and E. coli. Results obtained from our study strongly suggest that microcalorimetry is a fast, simple, and more sensitive technology that can be easily performed to study the effect of drugs on bacteria.
本文从制川乌中提取得到粗制乌头多糖，采用微量热法研究了粗制乌头多糖对大肠杆菌和金黄色葡萄球菌的作用。在 37°C 下，用 TAM air 等温微量热仪绘制了不同浓度（c）粗制乌头多糖作用下细菌生长的功率-时间曲线。计算了生长速率常数（μ）、抑制率（I）、峰高（P(m)）和峰时间（t(m)）。从这些数据中，还建立了μ与 c 之间的关系。随着粗制乌头多糖浓度的增加，生长速率常数μ降低。此外，随着浓度的增加，P(m)降低，t(m)增加。实验结果表明，粗制乌头多糖对金黄色葡萄球菌和大肠杆菌具有抑制活性。本研究结果强烈表明，微量热法是一种快速、简单、更灵敏的技术，可用于研究药物对细菌的作用。
