Huang Xueqing, Li Li, Huang Cui, Du Xijin
The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Eur J Oral Sci. 2011 Aug;119(4):310-5. doi: 10.1111/j.1600-0722.2011.00830.x. Epub 2011 Jun 15.
Frequently encountered in clinical practice, caries-affected dentine (CAD) is the most challenging bonding substrate. This study evaluated the effect of ethanol-wet bonding with hydrophobic adhesive to sound dentine and to CAD. In the control groups, prepared sound dentine and CAD were bonded with Adper Single Bond 2 using a traditional water-wet bonding technique. In the experimental groups, the specimens were treated as follows: Group 1, rinsed with stepwise ethanol dehydration; Group 2, immersion in 100% ethanol, three times, for 20 s each time; and Group 3, immersion in 100% ethanol for 20 s. Microtensile bond strength (μTBS) testing was used to evaluate the effects of the different protocols on bonding. The microhardness of debonded dentine surfaces was measured to ensure the presence of CAD. Interfacial nanoleakage was evaluated by field-emission scanning electron microscopy. Treatment significantly improved the μTBS in CAD in Groups 1 and 2, but had no effect on Group 3. Conversely, treatment significantly reduced the μTBS in sound dentine in Groups 2 and 3, but had no effect in Group 1. The presence of nanoleakage varied with the ethanol-wet protocol used. In conclusion, ethanol-wet bonding can potentially improve bond efficacy to CAD when an appropriate protocol is used.
龋坏牙本质（CAD）在临床实践中经常遇到，是最具挑战性的粘结基底。本研究评估了使用疏水粘结剂对健康牙本质和CAD进行乙醇湿粘结的效果。在对照组中，制备的健康牙本质和CAD采用传统的水湿粘结技术用Adper Single Bond 2进行粘结。在实验组中，标本按以下方式处理：第1组，用逐步乙醇脱水冲洗；第2组，浸入100%乙醇中，每次浸泡20秒，共浸泡3次；第3组，浸入100%乙醇中20秒。采用微拉伸粘结强度（μTBS）测试来评估不同方案对粘结的影响。测量脱粘牙本质表面的显微硬度以确保CAD的存在。通过场发射扫描电子显微镜评估界面纳米渗漏。处理显著提高了第1组和第2组CAD的μTBS，但对第3组没有影响。相反，处理显著降低了第2组和第3组健康牙本质的μTBS，但对第1组没有影响。纳米渗漏的存在因所使用的乙醇湿粘结方案而异。总之，当使用适当的方案时，乙醇湿粘结可能会提高对CAD的粘结效果。
