Silva Francisco Cláudio Fernandes Alves, Feitosa Victor Pinheiro, Saboia Vicente de Paulo Aragão
Department of Dentistry, Christus University Center, Fortaleza, Ceará, Brazil.
Department of Restorative Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, Ceará, Brazil.
Eur J Dent. 2019 May;13(2):137-142. doi: 10.1055/s-0039-1696584. Epub 2019 Sep 3.
The aim of this study was to evaluate the use of 70 vol.% ethanol as a dentin pretreatment on the bond strength (BS) of a two-step etch-and-rinse adhesive.
Resin composite Class I restorations were clinically bonded to acid-etched dentin of human sound third molars using Adper Single Bond 2 (SB, 3M ESPE) and randomly divided into two major groups: dentin saturated with water (control) or 70 vol.% ethanol (ethanol). The teeth were divided into two subgroups: immediately extracted and tested after 24 hours and extraction after 18 months. Bonded teeth (Adper SB 2) were cut into resin-dentin sticks that were tested by microtensile BS, and the failure mode was thereafter evaluated.
Data were statistically analyzed using two-way analysis of variance and Holm-Sidak test (α = 0.05). Additional bonded resin-dentin slabs from each group were examined under light microscopy (LM) using the Masson's trichrome staining technique.
The lowest BS was obtained by ethanol pretreated dentin after aging, while other groups presented similar BS. The LM analysis showed the presence of resin-sparse collagen fibrils in groups examined immediately (24 hours) and the presence of several gaps due to collagen degradation at the interfaces of ethanol pretreated aged specimens (18 months).
The use of 70 vol.% ethanol in dentin before the application of a two-step etch-and-rinse adhesive should be avoided once it resulted in a significant drop on the BS.
本研究旨在评估70%（体积分数）乙醇作为牙本质预处理剂对两步酸蚀冲洗粘结剂粘结强度（BS）的影响。
使用Adper Single Bond 2（SB，3M ESPE）将树脂复合体I类修复体临床粘结至健康人第三磨牙的酸蚀牙本质上，并随机分为两大组：用水饱和牙本质（对照组）或70%（体积分数）乙醇（乙醇组）。牙齿又分为两个亚组：立即拔除并在24小时后测试，以及在18个月后拔除。将粘结的牙齿（Adper SB 2）切成树脂-牙本质棒，通过微拉伸粘结强度进行测试，然后评估失败模式。
数据采用双向方差分析和Holm-Sidak检验进行统计学分析（α = 0.05）。每组额外的粘结树脂-牙本质板使用马松三色染色技术在光学显微镜（LM）下进行检查。
老化后乙醇预处理的牙本质获得的粘结强度最低，而其他组的粘结强度相似。光学显微镜分析显示，立即检查的组（24小时）中存在树脂稀疏的胶原纤维，而在乙醇预处理的老化标本界面（18个月）处，由于胶原降解存在多个间隙。
在应用两步酸蚀冲洗粘结剂之前，应避免在牙本质中使用70%（体积分数）乙醇，因为它会导致粘结强度显著下降。
