在波兰野生鸟类中检测西尼罗河病毒的尝试。

Attempts to detect West Nile virus in wild birds in Poland.

作者信息

Niczyporuk Jowita Samanta, Samorek-Salamonowicz Elżbieta, Kozdruń Wojciech, Mizak Zbigniew

机构信息

National Veterinary Research Institute Puławy, Department of Poultry Viral Diseases, Pulawy, Poland.

出版信息

Acta Vet Hung. 2011 Sep;59(3):405-8. doi: 10.1556/AVet.2011.023.

DOI:10.1556/AVet.2011.023

PMID:21727072

Abstract

The aim of the study was to attempt the detection of West Nile virus (WNV) in wild birds in Poland. Forty-eight species of 1912 wild birds were used for the investigations. The birds were derived from various locations in Poland from early spring till late autumn of the years 2009-2011. The brain samples were homogenised and cellular RNA was isolated. Two methods (RT-PCR and nested RT-PCR) were used. The presence of WNV RNA was not detected in the samples examined. Additionally, a short analysis of the epizootiological situation regarding the presence of WNV in Poland is presented.

摘要

该研究的目的是尝试在波兰的野生鸟类中检测西尼罗河病毒（WNV）。对1912只野生鸟类的48个物种进行了调查。这些鸟类于2009年至2011年从波兰各地采集，时间从早春到晚秋。将脑样本匀浆并分离细胞RNA。使用了两种方法（逆转录聚合酶链反应和巢式逆转录聚合酶链反应）。在所检测的样本中未检测到WNV RNA。此外，还对波兰西尼罗河病毒存在情况的动物流行病学状况进行了简要分析。