Deka Rekha, Kumar Ravi, Tamuli Ranjan
Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati 781 039, India.
Genetica. 2011 Jul;139(7):885-94. doi: 10.1007/s10709-011-9592-y. Epub 2011 Jul 5.
NCU04379 gene encodes a conserved Ca(2+) and/or calmodulin binding protein that possesses a consensus signal for N-terminal myristoylation and four EF-hands, characteristics of Neuronal Calcium Sensor-1proteins. The NCU04379.2 knockout mutant shows slow growth rate, increased sensitivity to calcium and ultraviolet (UV) irradiation, and a wild-type fragment carrying NCU04379 gene complements the mutant. Therefore, NCU04379 gene has a role in growth, calcium stress tolerance, and UV survival. Crosses homozygous for ΔNCU04379.2 mutant strains were fully fertile; however, we found evidence for involvement of Ca(2+)/calmodulin-dependent protein kinase encoding genes NCU02283 and NCU09123 in sexual development.
NCU04379基因编码一种保守的钙（Ca2+）和/或钙调蛋白结合蛋白，该蛋白具有N端肉豆蔻酰化的共有信号和四个EF手结构，这是神经元钙传感器-1蛋白的特征。NCU04379.2基因敲除突变体表现出生长速率缓慢、对钙和紫外线（UV）照射的敏感性增加，并且携带NCU04379基因的野生型片段可补充该突变体。因此，NCU04379基因在生长、钙胁迫耐受性和紫外线存活方面发挥作用。ΔNCU04379.2突变体菌株的纯合杂交完全可育；然而，我们发现编码基因NCU02283和NCU09123的钙（Ca2+）/钙调蛋白依赖性蛋白激酶参与了有性发育。
