用于分析与在线衍生化相容的前列腺特异性抗原同工型的 CE 方法，用于 LIF 检测。
CE methods for analysis of isoforms of prostate-specific antigen compatible with online derivatization for LIF detection.
机构信息
Institute of Organic Chemistry (CSIC), Madrid, Spain.
出版信息
Electrophoresis. 2011 Aug;32(15):2036-43. doi: 10.1002/elps.201000524. Epub 2011 Jul 4.
Prostate-specific antigen (PSA) is the usual biomarker for prostate cancer (PCa). However, its lack of selectivity has lead to the search for new biomarkers. PSA glycosylation seems to depend on the pathophysiological conditions of the individual. Thus, methods to separate PSA isoforms (peaks) to study their role as PCa markers are needed. In this work, CE methods for PSA isoforms separation, based on the use of different dynamic coatings, are developed using UV detection. Three complementary CE methods allowing the separation of 8 or 9 PSA isoforms are selected. The longest method takes only 17 min, while the shortest one separates 9 isoforms in < 8 min. Depending on the isoforms of interest for their use as PCa biomarker, the CE method to be used can be chosen or various of them can be combined. A remarkable aspect of these methods is that the BGEs employed are devoid of compounds with primary amino groups, making the CE methods compatible with fluorescent on-column derivatization through amino residues. As a proof-of-concept, a preliminary result shows that LIF detection of labeled PSA analyzed by one of the three developed methods permits detection of glycoprotein isoforms.
前列腺特异性抗原（PSA）是前列腺癌（PCa）的常用生物标志物。然而，其缺乏选择性导致了对新生物标志物的寻找。PSA 糖基化似乎取决于个体的病理生理状况。因此，需要分离 PSA 同工型（峰）的方法来研究它们作为 PCa 标志物的作用。在这项工作中，开发了基于使用不同动态涂层的 CE 方法来分离 PSA 同工型，并用 UV 检测。选择了三种互补的 CE 方法，可分离 8 或 9 种 PSA 同工型。最长的方法仅需 17 分钟，而最短的方法在 <8 分钟内分离 9 种同工型。根据将其用作 PCa 生物标志物的同工型的不同，可以选择要使用的 CE 方法，或者可以将它们组合使用。这些方法的一个显著特点是，所使用的 BGE 不含具有伯氨基的化合物，从而使 CE 方法与通过氨基酸残基进行柱上荧光衍生兼容。作为概念验证，初步结果表明，通过三种方法之一分析的标记 PSA 的 LIF 检测可检测糖蛋白同工型。
Zotero 插件