前列腺特异性抗原（PSA）的毛细管电泳分析
Capillary Electrophoresis Analysis of Prostate-Specific Antigen (PSA).
作者信息
Farina-Gomez Noemi, Navarro-Calderon Diana, Puerta Angel, Gonzalez Monica, Diez-Masa José Carlos, de Frutos Mercedes
机构信息
Institute of Organic Chemistry, (IQOG-CSIC), Madrid, Spain.
出版信息
Methods Mol Biol. 2019;1972:221-234. doi: 10.1007/978-1-4939-9213-3_16.
The Capillary Electrophoresis (CE) profile of isoforms (peaks) of a glycoprotein can be useful to show alterations in its posttranslational modifications (PTMs) linked to diseases. These changes can modify the electrophoretic mobility of these isoforms in a minor extent and, therefore, very reproducible CE methods are needed to detect them. In this chapter, a method for the analysis of prostate-specific antigen (PSA) by Capillary Zone Electrophoresis (CZE) with UV detection is detailed. High reproducibility in the separation of a large number of PSA isoforms is achieved by performing capillary conditioning in acid media and by using a background electrolyte (BGE) at pH 8.0 formulated with decamethonium bromide and urea.
糖蛋白异构体（峰）的毛细管电泳（CE）图谱有助于显示其与疾病相关的翻译后修饰（PTM）的改变。这些变化会在较小程度上改变这些异构体的电泳迁移率，因此，需要非常可重复的CE方法来检测它们。在本章中，详细介绍了一种采用紫外检测的毛细管区带电泳（CZE）分析前列腺特异性抗原（PSA）的方法。通过在酸性介质中进行毛细管预处理，并使用由溴化十烃季铵和尿素配制的pH 8.0的背景电解质（BGE），可在分离大量PSA异构体时实现高重现性。
Zotero 插件