Department of Radiation Oncology, University of Pittsburgh Cancer Institute, Pittsburgh, PA 15232, USA.
Int J Radiat Biol. 2011 Oct;87(10):1052-60. doi: 10.3109/09553002.2011.587860. Epub 2011 Jul 5.
To determine whether Carbamazepine (CBZ) is a radiation protector and/or mitigator.
Murine hematopoietic progenitor 32D cl 3 cells were incubated in 1, 10, or 100 μM CBZ 1 h before or immediately after 0-8 Gy irradiation and assayed for clonogenic survival. Autophagy was assayed by immunoblot for microtubule-associated protein light chain 3 (LC3). In vivo radioprotection and mitigation were determined with C57BL/6NTac mice.
CBZ treatment at 1, 10 or 100 μM for 1 h prior to irradiation increased radioresistance (the dose for 37% survival or D(0)) from control 1.5 ± 0.1 Gy to 2.1 ± 0.2 Gy (P = 0.012), 2.3 ± 0.1 Gy (P = 0.010), and 3.6 ± 0.7 Gy (P = 0.003), respectively; after irradiation increased the extrapolation number (ñ) from 1.5 ± 0.3 to 10.1 ± 4.2 (P = 0.011), 5.5 ± 1.7 (P = 0.019), and 3.6 ± 0.8 (P = 0.014), respectively, and increased autophagy. CBZ treated mice 10 min or 24 h before or 10 min or 12 h after 9.25 Gy total body irradiation (TBI) showed increased survival (P = 0.012, 0.011, 0.0002, and 0.017, respectively).
CBZ may be a useful radiation protector and mitigator.
确定卡马西平（CBZ）是否为辐射防护剂和/或缓解剂。
在 0-8Gy 照射前 1 小时或照射后立即，将 32D cl 3 细胞在 1、10 或 100μM 的 CBZ 中孵育，并检测集落形成细胞的存活情况。通过免疫印迹法检测微管相关蛋白轻链 3（LC3）来检测自噬。使用 C57BL/6NTac 小鼠进行体内辐射防护和缓解作用。
照射前 1、10 或 100μM 的 CBZ 处理 1 小时可提高放射抗性（37%存活剂量或 D(0)），从对照 1.5±0.1Gy 增加到 2.1±0.2Gy（P=0.012）、2.3±0.1Gy（P=0.010）和 3.6±0.7Gy（P=0.003）；照射后，将外推数（ñ）从 1.5±0.3 增加到 10.1±4.2（P=0.011）、5.5±1.7（P=0.019）和 3.6±0.8（P=0.014），并增加自噬。在 9.25Gy 全身照射（TBI）前 10 分钟或 24 小时、照射后 10 分钟或 12 小时给予 CBZ 的小鼠显示出存活率增加（P=0.012、0.011、0.0002 和 0.017）。
CBZ 可能是一种有用的辐射防护剂和缓解剂。
