Kondo Natsuko I, Tuda Midori, Toquenaga Yukihiko, Lan Yen-Chiu, Buranapanichpan Sawai, Horng Shwu-Bin, Shimada Masakazu, Fukatsu Takema
Environmental Biology, National Institute for Environmental Studies (NIES), 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
Zoolog Sci. 2011 Jul;28(7):501-8. doi: 10.2108/zsj.28.501.
Wolbachia endosymbionts are widespread among insects and other arthropods, often causing cytoplasmic incompatibility and other reproductive phenotypes in their hosts. Recently, possibilities of Wolbachia-mediated pest control and management have been proposed, and the bean beetles of the subfamily Bruchinae are known as serious pests of harvested and stored beans worldwide. Here we investigated Wolbachia infections in bean beetles from the world, representing seven genera, 20 species and 87 populations. Of 20 species examined, Wolbachia infections were detected in four species, Megabruchidius sophorae, Callosobruchus analis, C. latealbus and C. chinensis. Infection frequencies were partial in M. sophorae but perfect in the other species. In addition to C. chinensis described in the previous studies, C. latealbus was infected with two distinct Wolbachia strains. These Wolbachia strains from the bean beetles were phylogenetically not closely related to each other. Among world populations of C. chinensis, some Taiwanese populations on a wild leguminous plant, Rhynchosia minima, exhibited a peculiar Wolbachia infection pattern, suggesting the possibility that these populations comprise a distinct host race or a cryptic species.
沃尔巴克氏体共生菌在昆虫和其他节肢动物中广泛存在，常常在其宿主中引发细胞质不相容性及其他生殖表型。最近，有人提出了利用沃尔巴克氏体进行害虫防治和管理的可能性，而豆象亚科的豆象是全球范围内收获和储存豆类的严重害虫。在此，我们调查了来自世界各地的豆象中的沃尔巴克氏体感染情况，这些豆象代表了7个属、20个物种和87个种群。在所检测的20个物种中，在四纹豆象、赤拟谷盗、白纹豆象和绿豆象这四个物种中检测到了沃尔巴克氏体感染。在四纹豆象中感染频率是部分感染，但在其他物种中是完全感染。除了先前研究中描述的绿豆象外，白纹豆象还感染了两种不同的沃尔巴克氏体菌株。这些来自豆象的沃尔巴克氏体菌株在系统发育上彼此关系不密切。在绿豆象的全球种群中，一些生长在野生豆科植物鸡眼草上的台湾种群呈现出一种特殊的沃尔巴克氏体感染模式，这表明这些种群有可能构成一个独特的宿主族或一个隐存种。
