Sontowski Rebekka, Bernhard Detlef, Bleidorn Christoph, Schlegel Martin, Gerth Michael
Molecular Evolution and Systematics of Animals Institute for Biology University of Leipzig Talstrasse 33 D-04103 Leipzig Germany.
Molecular Evolution and Systematics of Animals Institute for Biology University of Leipzig Talstrasse 33 D-04103 Leipzig Germany ; German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig Deutscher Platz 5d 04103 Leipzig Germany.
Ecol Evol. 2015 Sep 16;5(19):4345-53. doi: 10.1002/ece3.1641. eCollection 2015 Oct.
Wolbachia (Alphaproteobacteria) is an inherited endosymbiont of arthropods and filarial nematodes and was reported to be widespread across insect taxa. While Wolbachia's effects on host biology are not understood from most of these hosts, known Wolbachia-induced phenotypes cover a spectrum from obligate beneficial mutualism to reproductive manipulations and pathogenicity. Interestingly, data on Wolbachia within the most species-rich order of arthropods, the Coleoptera (beetles), are scarce. Therefore, we screened 128 species from seven beetle families (Buprestidae, Hydraenidae, Dytiscidae, Hydrophilidae, Gyrinidae, Haliplidae, and Noteridae) for the presence of Wolbachia. Our data show that, contrary to previous estimations, Wolbachia frequencies in beetles (31% overall) are comparable to the ones in other insects. In addition, we used Wolbachia MLST data and host phylogeny to explore the evolutionary history of Wolbachia strains from Hydraenidae, an aquatic lineage of beetles. Our data suggest that Wolbachia from Hydraenidae might be largely host genus specific and that Wolbachia strain phylogeny is not independent to that of its hosts. As this contrasts with most terrestrial Wolbachia-arthropod systems, one potential conclusion is that aquatic lifestyle of hosts may result in Wolbachia distribution patterns distinct from those of terrestrial hosts. Our data thus provide both insights into Wolbachia distribution among beetles in general and a first glimpse of Wolbachia distribution patterns among aquatic host lineages.
沃尔巴克氏体（α-变形菌纲）是节肢动物和丝虫线虫的一种遗传性内共生菌，据报道在昆虫类群中广泛存在。虽然从大多数这些宿主中还不了解沃尔巴克氏体对宿主生物学的影响，但已知沃尔巴克氏体诱导的表型涵盖了从专性有益共生到生殖操纵和致病性的范围。有趣的是，在节肢动物中物种最丰富的目——鞘翅目（甲虫）中，关于沃尔巴克氏体的数据很少。因此，我们从七个甲虫科（吉丁甲科、沼甲科、龙虱科、水龟甲科、豉甲科、沼梭甲科和圆泥甲科）的128个物种中筛选了沃尔巴克氏体的存在情况。我们的数据表明，与之前的估计相反，甲虫中的沃尔巴克氏体感染率（总体为31%）与其他昆虫中的感染率相当。此外，我们利用沃尔巴克氏体多位点序列分型数据和宿主系统发育来探究来自沼甲科（甲虫的一个水生类群）的沃尔巴克氏体菌株的进化历史。我们的数据表明沼甲科的沃尔巴克氏体可能在很大程度上具有宿主属特异性，并且沃尔巴克氏体菌株的系统发育与其宿主的系统发育并非独立。由于这与大多数陆地沃尔巴克氏体-节肢动物系统形成对比，一个可能的结论是宿主的水生生活方式可能导致沃尔巴克氏体的分布模式与陆地宿主不同。因此，我们的数据既为沃尔巴克氏体在甲虫中的总体分布提供了见解，也首次揭示了水生宿主类群中沃尔巴克氏体的分布模式。
