Busse E, Kornhuber B
Zentrum der Kinderheilkunde, J. W. Goethe-Universität, Frankfurt.
Onkologie. 1990 Aug;13(4):301-4. doi: 10.1159/000216780.
The change of Adenylcyclase from the plasma membrane of neuroblastoma cells during cell differentiation was investigated. As a parameter for the change of the Adenylcyclase activity we determined the Adenylcyclase stimulatability caused by the neurotransmitters noradrenaline, dopamine and adrenaline. In differentiated neuroblastoma cells the basilar Adenylcyclase activity was higher than in undifferentiated cells. The tested neurotransmitters showed the same result. By means of a G-protein from medullary adrenal gland we succeeded in reconstructing the Adenylcyclase from both undifferentiated and differentiated cells. The importance of the Gs protein for the cell differentiation is discussed.
研究了神经母细胞瘤细胞质膜中腺苷酸环化酶在细胞分化过程中的变化。作为腺苷酸环化酶活性变化的一个参数，我们测定了神经递质去甲肾上腺素、多巴胺和肾上腺素引起的腺苷酸环化酶刺激能力。在分化的神经母细胞瘤细胞中，基础腺苷酸环化酶活性高于未分化细胞。所测试的神经递质也显示出相同的结果。借助于来自肾上腺髓质的一种G蛋白，我们成功地重建了未分化和分化细胞中的腺苷酸环化酶。讨论了Gs蛋白在细胞分化中的重要性。
