Amer H E, Asker S A, Mazroa S A
Department of Otorhinolaryngology, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
J Laryngol Otol. 2011 Aug;125(8):829-36. doi: 10.1017/S0022215111000831. Epub 2011 May 13.
Anabolic steroid abuse by women is associated with a number of adverse effects, including laryngeal changes. The epidermal growth factor receptor is related to regulation of the cell life cycle. This study aimed to investigate the structural changes and immunohistochemical localisation of epidermal growth factor receptor in rat vocal folds following anabolic steroid administration, and also to assess the effect of anti-androgens.
Thirty-two adult female albino rats were divided into: group I (controls), group II (receiving anabolic steroids for two months) or group III (receiving anabolic steroids plus anti-androgen for two months).
Group II rat true vocal folds showed thicker epithelial layers with many mitotic figures, thicker lamina propria and thicker muscle fibres; epithelial cells were also immunohistochemically positive for epidermal growth factor receptor. Group III rats showed similar changes, but thin muscle fibres and extravasated red blood cells within the lamina propria.
Anabolic steroids caused structural and immunohistochemical changes within the female rat true vocal fold. Co-administration of anti-androgens did not prevent these changes, suggesting that anti-androgens have a limited role in the management of such changes in humans.
女性滥用合成代谢类固醇与包括喉部变化在内的多种不良反应相关。表皮生长因子受体与细胞生命周期的调控有关。本研究旨在探讨合成代谢类固醇给药后大鼠声带中表皮生长因子受体的结构变化和免疫组化定位，并评估抗雄激素的作用。
将32只成年雌性白化大鼠分为：第一组（对照组）、第二组（接受合成代谢类固醇两个月）或第三组（接受合成代谢类固醇加抗雄激素两个月）。
第二组大鼠的真声带显示上皮层增厚，有许多有丝分裂象，固有层增厚，肌纤维增厚；上皮细胞免疫组化检测表皮生长因子受体也呈阳性。第三组大鼠表现出类似变化，但肌纤维较细，固有层内有红细胞外渗。
合成代谢类固醇导致雌性大鼠真声带出现结构和免疫组化变化。联合使用抗雄激素并不能预防这些变化，这表明抗雄激素在人类此类变化的管理中作用有限。
