从菊科朝鲜橐吾中分离和鉴定 10 个微卫星位点。
Isolation and characterization of 10 microsatellite loci from Korean Leontopodium japonicum (Asteraceae).
机构信息
Department of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
出版信息
Am J Bot. 2011 Jul;98(7):e183-4. doi: 10.3732/ajb.1100065. Epub 2011 Jun 17.
PREMISE OF THE STUDY
Microsatellite primers were developed for the alpine plant Leontopodium japonicum to evaluate its population genetics structure as a means for devising future conservation strategies.
METHODS AND RESULTS
Ten polymorphic microsatellite markers were developed in Korean populations of L. japonicum. Genetic diversity was analyzed among 38 individuals from two populations. Overall, each locus had 3 to 17 alleles, and their observed and expected heterozygosities ranged from 0.0000 to 0.8750 and from 0.0000 to 0.8929, respectively.
CONCLUSIONS
The microsatellite markers described here are valuable tools for population genetics research of L. japonicum. They can be used to obtain information about suitable management strategies for protecting other related and endangered "edelweiss" species.
研究前提
为高山植物橐吾属（Leontopodium japonicum）开发微卫星引物，以评估其种群遗传结构，作为制定未来保护策略的一种手段。
方法与结果
在韩国的橐吾属种群中开发了 10 个多态性微卫星标记。对来自两个种群的 38 个个体进行了遗传多样性分析。总的来说，每个位点有 3 到 17 个等位基因，其观测和预期杂合度范围分别为 0.0000 到 0.8750 和 0.0000 到 0.8929。
结论
这里描述的微卫星标记是研究橐吾属种群遗传学的有价值的工具。它们可用于获取有关保护其他相关和濒危“雪绒花”物种的合适管理策略的信息。
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