开发濒危药用植物垂头菊（菊科）的微卫星标记。
Development of microsatellite markers for the endangered medicinal plant Launaea arborescens (Asteraceae).
机构信息
Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, People's Republic of China.
出版信息
Am J Bot. 2012 Dec;99(12):e481-3. doi: 10.3732/ajb.1200126. Epub 2012 Nov 28.
PREMISE OF THE STUDY
Microsatellite loci were developed in Launaea arborescens, an endangered and medicinal Asteraceae species in North Africa, for further investigation of its conservation genetics. •
METHODS AND RESULTS
We isolated and characterized 10 polymorphic and nine monomorphic microsatellite loci from L. arborescens using the protocol of Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO). For the 10 polymorphic loci, the number of alleles detected per locus varied from two to six, and the observed and expected heterozygosities ranged from 0.000 to 0.833 and 0.059 to 0.713, respectively. •
CONCLUSIONS
The polymorphic markers provide a useful tool for conservation genetics studies of L. arborescens, including analysis of mating system, estimating gene flow, and identifying discrete genetic units within the species.
研究前提
在北非一种濒危药用菊科植物——肋柱花中开发了微卫星位点，以进一步研究其保护遗传学。
方法和结果
我们使用序列中重复快速分离 AFLP（FIASCO）的方案，从肋柱花中分离并鉴定了 10 个多态性和 9 个单态性微卫星位点。对于 10 个多态性位点，每个位点检测到的等位基因数从两个到六个不等，观察到的和预期的杂合度分别在 0.000 到 0.833 和 0.059 到 0.713 之间。
结论
多态性标记为肋柱花的保护遗传学研究提供了有用的工具，包括交配系统分析、估计基因流以及识别物种内的离散遗传单位。
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