Meirelles S L, Gouveia G V, Gasparin G, Alencar M M, Gouveia J J S, Regitano L C A
Departamento de Genética e Evolução, Universidade Federal de São Carlos, São Carlos, SP, Brasil.
Genet Mol Res. 2011 Jun 21;10(2):1220-6. doi: 10.4238/vol10-2gmr1175.
Investigation of molecular marker effects on production traits is essential to define marker assisted selection strategies in beef cattle. We looked for a possible association of molecular markers and backfat thickness (BFT) and rib eye area (REA) in Canchim (5/8 Charolais + 3/8 Zebu) and MA (offspring of Charolais bulls and 1/2 Canchim + 1/2 Zebu cows) animals raised exclusively on pasture. Traits were measured on 987 individuals from seven herds from two Brazilian States (São Paulo and Goiás), in March and April from 2005 to 2007, when animals were, on average, 19 months of age. Five microsatellite markers lying in QTL regions for BFT and REA (BMS490 and ETH10 on chromosome 5, INRA133 and ILSTS090 on chromosome 6, and BMS2142 on chromosome 19) were genotyped and association analyses were performed under an animal model using the restricted maximum likelihood method. After correction for multiple tests, a significant effect of microsatellite BMS490 on REA was observed, suggesting that at least one QTL affecting carcass traits in this region of the BTA5. No significant effect on BFT was observed for these markers.
研究分子标记对生产性状的影响对于确定肉牛的标记辅助选择策略至关重要。我们在完全以牧场饲养的坎辛（5/8夏洛莱 + 3/8泽布）和MA（夏洛莱公牛与1/2坎辛 + 1/2泽布母牛的后代）动物中，寻找分子标记与背膘厚度（BFT）和眼肌面积（REA）之间的可能关联。在2005年至2007年的3月和4月，对来自巴西两个州（圣保罗和戈亚斯）七个牛群的987头个体进行了性状测量，此时动物平均年龄为19个月。对位于BFT和REA的QTL区域的五个微卫星标记（5号染色体上的BMS490和ETH10、6号染色体上的INRA133和ILSTS090以及19号染色体上的BMS2142）进行基因分型，并使用限制最大似然法在动物模型下进行关联分析。在对多重检验进行校正后，观察到微卫星BMS490对REA有显著影响，这表明在BTA5的该区域至少有一个影响胴体性状的QTL。未观察到这些标记对BFT有显著影响。
