Zhao Yuan, Zhang Xiaoxue, Li Fadi, Zhang Deyin, Zhang Yukun, Li Xiaolong, Song Qizhi, Zhou Bubo, Zhao Liming, Wang Jianghui, Xu Dan, Cheng Jiangbo, Li Wenxin, Lin Changchun, Yang Xiaobin, Zeng Xiwen, Wang Weimin
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, China.
The State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China.
Front Genet. 2022 Mar 14;13:824742. doi: 10.3389/fgene.2022.824742. eCollection 2022.
In sheep meat production, the rib eye area is an important index to evaluate carcass traits. However, conventional breeding programs have led to slow genetic progression in rib eye muscle area. Operationalizing molecular marker assisted breeding is an optimized breeding method that might improve this situation. Therefore, the present study used whole genome sequencing data to excavate candidate genes associated with the rib eye muscle. Male Hu lambs ( = 776) with pedigrees and 274 lambs with no pedigree were included. The genetic parameters of the rib eye area were estimated using a mixed linear mixed model. The rib eye area showed medium heritability (0.32 ± 0.13). Whole-genome sequencing of 40 large rib eye sheep [17.97 ± 1.14, (cm)] and 40 small rib eye sheep [7.89 ± 0.79, (cm)] was performed. Case-control genome-wide association studies and the fixation index identified candidate rib eye-associated genes. Seven single nucleotide polymorphisms (SNPs) in six genes (, , , , , and ) were identified as candidates. The study population was expanded to 1050 lambs to perform KASPar genotyping on five SNPs, which demonstrated that SNPs in , , and correlated significantly with the rib eye area, which could be used as genetic markers for molecular breeding of the rib eye area. The results provided genetic parameters estimated on the rib eye area and information for breeding based on carcass traits in Hu sheep.
在绵羊肉生产中，肋眼面积是评估胴体性状的一个重要指标。然而，传统的育种计划导致肋眼肌面积的遗传进展缓慢。实施分子标记辅助育种是一种优化的育种方法，可能会改善这种情况。因此，本研究利用全基因组测序数据挖掘与肋眼肌相关的候选基因。纳入了有系谱的雄性湖羊羔羊（n = 776）和无系谱的274只羔羊。使用混合线性模型估计肋眼面积的遗传参数。肋眼面积显示出中等遗传力（0.32±0.13）。对40只大肋眼羊[17.97±1.14，（厘米）]和40只小肋眼羊[7.89±0.79，（厘米）]进行了全基因组测序。病例对照全基因组关联研究和固定指数确定了与肋眼相关的候选基因。在六个基因（，，，，，和）中鉴定出七个单核苷酸多态性（SNP）作为候选基因。将研究群体扩大到1050只羔羊，对五个SNP进行KASPar基因分型，结果表明，，和中的SNP与肋眼面积显著相关，可作为肋眼面积分子育种的遗传标记。研究结果提供了肋眼面积的遗传参数估计值以及基于湖羊胴体性状的育种信息。
