Edbauer C, Weinberg R, Taylor J, Rey-Senelonge A, Bouquet J F, Desmettre P, Paoletti E
Virogenetics Corporation, Troy, New York 12180.
Virology. 1990 Dec;179(2):901-4. doi: 10.1016/0042-6822(90)90165-n.
A recombinant fowlpox virus expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) strain Texas was generated. Immunoprecipitation with chicken anti-NDV serum confirmed authentic expression of the HN protein. Protection of chickens from infection with NDV was observed when birds were immunized with the recombinant HN fowlpox virus by the intramuscular route after one or two inoculations. Vaccination by the ocular route with a mixture of fowlpox recombinants expressing the fusion and HN proteins did not show added protection over that seen with the individual viruses.
构建了一种表达新城疫病毒（NDV）德克萨斯株血凝素神经氨酸酶（HN）蛋白的重组禽痘病毒。用鸡抗NDV血清进行免疫沉淀证实了HN蛋白的真实表达。当鸡通过肌肉注射途径用重组HN禽痘病毒进行一次或两次接种免疫后，观察到其对NDV感染具有保护作用。用表达融合蛋白和HN蛋白的禽痘重组体混合物通过眼内途径接种，并未显示出比单独使用病毒时更强的保护作用。
