Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
Antiviral Res. 2014 Jan;101:1-11. doi: 10.1016/j.antiviral.2013.10.016. Epub 2013 Nov 5.
In this study, the regions in the infectious bursal disease virus (IBDV) genome that are amenable to the introduction of a sequence encoding a virus-neutralizing epitope of Newcastle disease virus (NDV) hemagglutinin-neuraminidase (HN) protein were identified. By using the reverse genetics approach, insertions or substitutions of sequences encoding the NDV epitope were engineered in the exposed loops (PBC, PHI and [Formula: see text] ) of the VP2 capsid protein and the N terminus of the nonstructural VP5 protein as well as the pep7a and pep7b regions of the pVP2 precursor of a commonly used IBDV vaccine strain, Gt. Three recombinant IBDVs expressing the NDV epitopes were successfully rescued in the PBC, pep7b and VP5 regions and the expressed epitope was recognized by anti-HN antibodies. Genetic analysis showed that the IBDV recombinants carrying the NDV epitopes were stable in cell cultures and in chickens. Animal studies demonstrated that the IBDV recombinants were innocuous in chickens. Vaccination with the recombinant viruses generated antibody responses against both IBDV and NDV, and provided 70-80% protection against IBDV and 50-60% protection against NDV. These results indicate that the recombinant IBDV has the potential to serve as a novel vaccine vector for other pathogens. In future studies, it is worth considering research to improve IBDV vector vaccine to get complete protection and safety of animals and humans.
在这项研究中，鉴定了传染性法氏囊病病毒（IBDV）基因组中可引入编码新城疫病毒（NDV）血凝素-神经氨酸酶（HN）蛋白病毒中和表位序列的区域。通过使用反向遗传学方法，在常用的 IBDV 疫苗株 Gt 的 VP2 衣壳蛋白暴露环（PBC、PHI 和 [Formula: see text]）以及非结构 VP5 蛋白的 N 末端以及 pVP2 前体的 pep7a 和 pep7b 区域中，对编码 NDV 表位的序列进行了插入或取代。成功拯救了在 PBC、pep7b 和 VP5 区域表达 NDV 表位的三种重组 IBDV，并通过抗 HN 抗体识别表达的表位。遗传分析表明，携带 NDV 表位的 IBDV 重组体在细胞培养物和鸡中稳定。动物研究表明，携带 NDV 表位的 IBDV 重组体对鸡是无害的。用重组病毒接种可产生针对 IBDV 和 NDV 的抗体反应，并提供针对 IBDV 的 70-80%保护和针对 NDV 的 50-60%保护。这些结果表明，重组 IBDV 有可能成为其他病原体的新型疫苗载体。在未来的研究中，值得考虑研究改进 IBDV 载体疫苗，以获得动物和人类的完全保护和安全性。
