Stvolinskaia N S, Goncharenko T M, Krylova O Iu, Nikulina S E, Poliakova E D, Korovkin B F
Vopr Med Khim. 1990 Jul-Aug;36(4):60-2.
Effects of insulin was studied in the monolayer hepatocyte cultures of new born rats under anoxic conditions. Insulin 10(-7) M stabilized lysosomal membranes obtained after 20 min anoxia. I hr incubation of hepatocytes with insulin in normal conditions, which was followed by anoxia, caused a significant increase of acid phosphatase activity in the fraction enriched with lysosomes. Insulin caused more distinct stabilization effect on lysosomal membranes when its action was prolonged. On the other hand, insulin caused more than 2-fold increase of cAMP content in hepatocytes within 2 min of exposition as compared with control cultures. When exposition of the cells with insulin exceeded 2 min, lowering of cAMP content was observed. These data appear to indicate that stabilizing effect of insulin was secondary towards cAMP level increase. Indirect connection between cAMP level, insulin stabilizing action and the state of lysosomal membranes were noted in the rat hepatocyte culture.
在缺氧条件下，对新生大鼠单层肝细胞培养物中胰岛素的作用进行了研究。10(-7)M的胰岛素可稳定缺氧20分钟后获得的溶酶体膜。在正常条件下，肝细胞与胰岛素孵育1小时后再进行缺氧处理，导致富含溶酶体的组分中酸性磷酸酶活性显著增加。当胰岛素的作用延长时，其对溶酶体膜的稳定作用更为明显。另一方面，与对照培养物相比，胰岛素作用2分钟内可使肝细胞内cAMP含量增加2倍以上。当细胞与胰岛素的孵育时间超过2分钟时，观察到cAMP含量降低。这些数据似乎表明，胰岛素的稳定作用是cAMP水平升高的继发效应。在大鼠肝细胞培养物中，注意到cAMP水平、胰岛素稳定作用和溶酶体膜状态之间的间接联系。
