Korovkin B F, Poliakova E D, Stvolinskaia N S, Gerasimova Ts I, Ivanova T N
Biull Eksp Biol Med. 1988 Apr;105(4):417-9.
Acid phosphatase activity, ATP and cyclic nucleotide levels were studied in primary cardiomyocyte cultures of newborn rats under hypoxic conditions. It was shown that incubation of cardiac cells in Eagle's medium supplemented with 10% calf serum during hypoxia (5% O2, 5% CO2, 90% N2) caused an hour later a decrease in lysosomal acid phosphatase activity, whereas free enzyme activity increased. The exposure of cells in Hanks salt solution to one hour hypoxia resulted in a 3-fold decrease of ATP level and further increase in free acid phosphatase activity. It was found that cAMP and cGMP levels do not depend on the presence of hypoxia. The mechanisms involved in the regulation of lysosomal membrane state are discussed.
在缺氧条件下，对新生大鼠原代心肌细胞培养物中的酸性磷酸酶活性、三磷酸腺苷（ATP）和环核苷酸水平进行了研究。结果表明，在缺氧（5%氧气、5%二氧化碳、90%氮气）期间，将心脏细胞在补充有10%小牛血清的伊格尔培养基中孵育一小时后，溶酶体酸性磷酸酶活性降低，而游离酶活性增加。将细胞置于汉克斯盐溶液中暴露于一小时缺氧状态会导致ATP水平降低三倍，并使游离酸性磷酸酶活性进一步增加。发现环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）水平不依赖于缺氧的存在。文中讨论了参与溶酶体膜状态调节的机制。
