Nikulina S E, Krylova O Iu, Goncharenko T M, Stvolinskaia N S, Poliakova E D, Korovkin B F
Vopr Med Khim. 1989 Sep-Oct;35(5):103-8.
Acid phosphatase activity and cAMP level were studied in primary hepatocyte culture of new born rats under conditions of anoxia and substrate deprivation (incubation of the cells in Hanks salt solution). Incubation of hepatocytes in Hanks salt solution within one hour under conditions of anoxia caused a significant increase in free (cytosolic) enzyme activity. Substitution of Hanks salt solution by normal tissue culture medium and reoxygenation after 1 hr anoxia resulted in a decrease of free acid phosphatase activity, whereas activity of the enzyme in lysosomal fraction was increased. Content of cAMP was decreased distinctly after 15 min incubation of hepatocyte culture under conditions of anoxia and substrate deprivation and was increased above control values within 5 min of reoxygenation and substitution of Hanks salt solution by normal tissue culture media. Addition of cAMP-containing liposomes to hepatocyte culture under these experimental conditions led to a decrease in free acid phosphatase activity and to an increase of the enzyme activity in lysosomal fraction. cAMP appears to modulate the lability of hepatocyte lysosomal membrane. The mechanisms involved in these processes are discussed.
在缺氧和底物剥夺（细胞在汉克斯盐溶液中孵育）条件下，对新生大鼠原代肝细胞培养物中的酸性磷酸酶活性和环磷酸腺苷（cAMP）水平进行了研究。在缺氧条件下，将肝细胞在汉克斯盐溶液中孵育1小时，导致游离（胞质）酶活性显著增加。用正常组织培养基替代汉克斯盐溶液并在缺氧1小时后复氧，导致游离酸性磷酸酶活性降低，而溶酶体部分中该酶的活性增加。在缺氧和底物剥夺条件下，肝细胞培养物孵育15分钟后，cAMP含量明显降低，在复氧以及用正常组织培养基替代汉克斯盐溶液后5分钟内，cAMP含量升高至对照值以上。在这些实验条件下，向肝细胞培养物中添加含cAMP的脂质体导致游离酸性磷酸酶活性降低，溶酶体部分中该酶的活性增加。cAMP似乎调节肝细胞溶酶体膜的稳定性。讨论了这些过程中涉及的机制。
