Yamate J, Tajima M, Togo M, Shibuya N, Shibuya K, Kudow S
Nippon Institute for Biological Science, Tokyo, Japan.
Acta Pathol Jpn. 1990 Aug;40(8):554-61.
A cis-diamminedichloroplatinum (CDDP)-selected cell line (MT-R10) was induced by continuous exposure of an in vitro passaged cell line (MT-P) established from a transplantable rat malignant fibrous histiocytoma (MFH-MT) to CDDP. MT-R10, capable of proliferating in the presence of 1.0 microgram CDDP/ml, was passaged in CDDP-free medium. The doubling time of MT-R10 at passage 10 (MT-R10/10) was almost the same as that of MT-P, being 22.3 and 25.5 h, respectively. The concentration of CDDP required for 50% inhibition of MT-R10/10 proliferation was two-fold higher than that of MT-P. MT-R10 consisted of round, epithelial-type cells arranged in compact sheets. Ultrastructurally, MT-R10 had numerous free ribosomes, some mitochondria, and other poorly developed cytoplasmic organelles suggesting its undifferentiated nature. MT-R10 showed no reaction for acid phosphatase or non-specific esterase. Tumors induced in syngeneic rats by inoculation with MT-R10 consisted of small, round, undifferentiated cells with scanty cytoplasm. They showed organoid and trabecular patterns, and were often arranged in compact sheets. The neoplastic cells showed no reaction for any of the histiocytic lysosomal and antigenic markers tested, but exhibited a strong reaction for alkaline phosphatase. Bone formation was often observed in the tumors. These observations suggest that CDDP-selected, undifferentiated cells may have osteogenic potential and may be one of the progenitor cells of MFH-MT.
一种顺二氯二氨铂（CDDP）筛选的细胞系（MT-R10）是通过将从可移植大鼠恶性纤维组织细胞瘤（MFH-MT）建立的体外传代细胞系（MT-P）持续暴露于CDDP诱导产生的。MT-R10能够在含有1.0微克CDDP/毫升的培养基中增殖，并在不含CDDP的培养基中传代。第10代MT-R10（MT-R10/10）的倍增时间与MT-P几乎相同，分别为22.3小时和25.5小时。抑制MT-R10/10增殖50%所需的CDDP浓度比MT-P高两倍。MT-R10由呈紧密片状排列的圆形上皮样细胞组成。超微结构上，MT-R10有大量游离核糖体、一些线粒体和其他发育不良的细胞质细胞器，表明其未分化的性质。MT-R10对酸性磷酸酶或非特异性酯酶无反应。用MT-R10接种同基因大鼠诱导产生的肿瘤由小的、圆形的、未分化的细胞组成，细胞质稀少。它们呈现出器官样和小梁状模式，并且常常排列成紧密的片状。肿瘤细胞对所检测的任何组织细胞溶酶体和抗原标志物均无反应，但对碱性磷酸酶有强烈反应。在肿瘤中经常观察到骨形成。这些观察结果表明，CDDP筛选的未分化细胞可能具有成骨潜能，并且可能是MFH-MT的祖细胞之一。
