Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
Pathol Int. 1996 Aug;46(8):557-67. doi: 10.1111/j.1440-1827.1996.tb03654.x.
The histogenesis of malignant fibrous histiocytoma (MFH) was studied using cisplatin (CDDP)-resistant MT-R8 and MT-R9 cells derived from cloned undifferentiated MT-8 and fibrohistiocytic MT-9 cells, respectively, which had been established from transplantable rat MFH. CDDP concentrations required for 50% suppression of proliferation of MT-R8 and MT-R9 cells were 5.4- and 3.3-fold greater than those of parental MT-8 and MT-9, respectively. MT-R8 and MT-R9 showed the higher positive rates to histiocytic lysosomal/ antigenic (ED1 and ED2) markers. The number of alpha-smooth muscle actin (SMA)-positive cells significantly increased in MT-R8; SMA-positive cells were also observed in MT-R9, but no difference was seen between MT-9 and MT-R9. MT-R8 and MT-R9 expressed both histiocytic and myofibroblastic phenotypes. However, the histology of subcutaneous tumors induced in syngeneic rats by MT-R8 and MR-R9 did not always reflect their in vitro nature. MT-R8 developed undifferentiated sarcomas similar to parental MT-8 tumors. In contrast, MT-R9 induced tumors with polytypic histologies such as the storiform growth pattern, neoplastic growth of granular cells and myofibroblasts, osteosarcoma-like areas, collagen-rich areas containing well-developed fibroblasts and areas involving many lipoblasts. These in vivo observations suggest the multidirectional differentiation of MT-R9 cells. Phenotypic modulation of rat MFH cells seemed to be easily induced by CDDP. A possible histogenesis of MFH was discussed based on the data collected.
使用分别从克隆的未分化MT - 8细胞和纤维组织细胞性MT - 9细胞衍生而来的顺铂（CDDP）耐药MT - R8和MT - R9细胞，研究恶性纤维组织细胞瘤（MFH）的组织发生，MT - 8和MT - 9细胞是从可移植大鼠MFH中建立的。抑制MT - R8和MT - R9细胞增殖50%所需的CDDP浓度分别比亲代MT - 8和MT - 9细胞高5.4倍和3.3倍。MT - R8和MT - R9对组织细胞溶酶体/抗原（ED1和ED2）标志物的阳性率更高。MT - R8中α - 平滑肌肌动蛋白（SMA）阳性细胞数量显著增加；MT - R9中也观察到SMA阳性细胞，但MT - 9和MT - R9之间没有差异。MT - R8和MT - R9均表达组织细胞和成肌纤维细胞表型。然而，MT - R8和MR - R9在同基因大鼠中诱导的皮下肿瘤组织学并不总是反映其体外特性。MT - R8形成了与亲代MT - 8肿瘤相似的未分化肉瘤。相反，MT - R9诱导的肿瘤具有多种组织学类型，如席纹状生长模式、颗粒细胞和成肌纤维细胞的肿瘤性生长、骨肉瘤样区域、含有发育良好的成纤维细胞的富含胶原区域以及涉及许多脂肪母细胞的区域。这些体内观察结果提示MT - R9细胞的多向分化。大鼠MFH细胞的表型调节似乎很容易被CDDP诱导。基于收集到的数据，讨论了MFH可能的组织发生。
