Yamate J, Tajima M, Shibuya K, Kuwamura M, Kotani T, Sakuma S
Department of Veterinary Pathology, College of Agriculture, Osaka Prefecture University, Japan.
J Vet Med Sci. 1996 Oct;58(10):1017-20. doi: 10.1292/jvms.58.10_1017.
To investigate a possible phenotypic modulation, MT-8L and MT-9L cells were induced by in vitro culture of undifferentiated MT-8 and fibrohistiocytic MT-9 cells, which had been established from a rat malignant fibrous histiocytoma (MFH), in the medium containing 10 micrograms lipopolysaccharide (LPS)/ml. MT-8L and MT-9L gave greater positive reactions for histiocytic lysosomal markers and showed ultrastructures of histiocytic natures. In MT-8L, alpha-smooth muscle actin-positive myofibroblastic cells also significantly increased in number. MT-8L expressed both histiocytic and myofibroblastic phenotypes. MT-8L-induced tumors consisted mainly of storiform type MFH, differing from undifferentiated sarcoma type induced by MT-8. MT-9L and MT-9 tumors showed a storiform pattern. A phenotypic modulation of MFH cells was easily induced by LPS treatment.
为研究可能的表型调节，将未分化的MT - 8细胞和源自大鼠恶性纤维组织细胞瘤（MFH）的纤维组织细胞性MT - 9细胞在含有10微克脂多糖（LPS）/毫升的培养基中进行体外培养，从而诱导出MT - 8L和MT - 9L细胞。MT - 8L和MT - 9L对组织细胞溶酶体标记物呈现更强的阳性反应，并显示出具有组织细胞性质的超微结构。在MT - 8L中，α - 平滑肌肌动蛋白阳性的肌成纤维细胞数量也显著增加。MT - 8L表达组织细胞和肌成纤维细胞两种表型。MT - 8L诱导形成的肿瘤主要由席纹状型MFH组成，这与MT - 8诱导形成的未分化肉瘤型不同。MT - 9L和MT - 9肿瘤呈现席纹状模式。通过LPS处理可轻易诱导MFH细胞发生表型调节。
