Universidade Federal de Minas Gerais, UFMG, Escola de Veterinária, Departamento de Medicina Veterinária Preventiva, Laboratório de Sanidade de Ovinos e Caprinos, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
Vet Microbiol. 2011 Dec 15;153(3-4):299-306. doi: 10.1016/j.vetmic.2011.06.002. Epub 2011 Jun 15.
Caseous lymphadenitis is an infectious sheep and goats disease caused by Corynebacterium pseudotuberculosis and characterized by abscesses in superficial and visceral lymph nodes. C. pseudotuberculosis strains isolated from these hosts have been shown to be very difficult to type by the existing methods. The aim of this study is evaluating the potential of the Enterobacterial Repetitive Intergenic Consensus (ERIC-PCR) as a tool for molecular typing of C. pseudotuberculosis strains isolated in sheep. One hundred and twenty seven isolates of C. pseudotuberculosis were isolated from lesions suspected to have had caseous lymphadenitis collected from sheep at the slaughterhouse. Animals were from 24 flocks in 13 municipalities of the Minas Gerais State, Brazil. Species identification of the isolates was performed by routine biochemical tests and mPCR. Fingerprint was performed by RAPD using ERIC-1R, ERIC-2 and ERIC-1R+ERIC-2 primers. Seventeen different genotypes were generated by ERIC 1-PCR, 21 genotypes by ERIC 2-PCR and 21 genotypes by ERIC 1+2-PCR. Hunter-Gaston Discrimination Index (HGDI) found for ERIC 1, ERIC 2, ERIC 1+2 PCR were 0.69, 0.87, and 0.84, respectively. For most herds evaluated observed at most three different genotypes among isolates from animals of these property, in all ERIC-PCR assays. However a few flocks observed between four and nine genotypes per flock. The W Kendall value found for correlation among the three techniques of ERIC-PCR was 0.91 (P<5.0 x 10(-6)). The results show that ERIC-PCR has good discriminatory power and advantages over other DNA-based typing methods, making it a useful tool to discriminate C. pseudotuberculosis isolates.
干酪性淋巴结炎是一种感染绵羊和山羊的疾病，由假结核棒状杆菌引起，其特征是在浅表和内脏淋巴结中形成脓肿。从这些宿主中分离出的假结核棒状杆菌菌株已被证明很难用现有的方法进行分型。本研究旨在评估肠细菌重复基因间一致性（ERIC-PCR）作为绵羊假结核棒状杆菌菌株分子分型工具的潜力。从屠宰场怀疑患有干酪性淋巴结炎的病变中分离出 127 株假结核棒状杆菌。动物来自巴西米纳斯吉拉斯州 13 个城市的 24 个羊群。通过常规生化试验和 mPCR 进行分离物的种属鉴定。使用 ERIC-1R、ERIC-2 和 ERIC-1R+ERIC-2 引物通过 RAPD 进行指纹图谱分析。通过 ERIC 1-PCR 产生了 17 种不同的基因型，通过 ERIC 2-PCR 产生了 21 种基因型，通过 ERIC 1+2-PCR 产生了 21 种基因型。ERIC 1、ERIC 2、ERIC 1+2 PCR 的 Hunter-Gaston 鉴别指数（HGDI）分别为 0.69、0.87 和 0.84。在所评估的大多数牛群中，在所评估的动物的分离物中，在所有 ERIC-PCR 检测中，观察到最多三种不同的基因型。然而，少数牛群每群观察到 4 到 9 种基因型。在三种 ERIC-PCR 技术之间发现的 Kendall 相关性 W 值为 0.91（P<5.0 x 10(-6))。结果表明，ERIC-PCR 具有良好的鉴别能力，优于其他基于 DNA 的分型方法，是鉴别假结核棒状杆菌分离株的有用工具。
