Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt.
Department of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Matrouh, Egypt.
Appl Microbiol Biotechnol. 2022 Sep;106(17):5701-5713. doi: 10.1007/s00253-022-12102-x. Epub 2022 Aug 10.
A total no. of 65 Salmonella enterica isolates recovered from food samples, feces of diarrheic calves, poultry, and hospital patient in large five cities at Northern West Egypt were obtained from the Department of Microbiology, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt. The 65 Salmonella enterica isolates had the invA gene were grouped into 11 Salmonella enterica serovars with dominance of S. Enteritidis and S. Kentucky serovars. Their resistance pattern were characterized by using 18 antibiotics from different classes. Approximately 80% of the isolates were multidrug resistant (MDR). Enterobacterial repetitive intergenic consequences polymerase chain reaction (ERIC-PCR) typing of 7 strains of S. Enteritidis showed 5 clusters with dissimilarity 25%. S. Enteritidis clusters in 2 main groups A and B. Group A have 2 human strain (HE2 and HE3) and one food origin (FE7) with a similarity 99%. Group B divided into B1 (FE2) and B2 (FE3) with a similarity ratio ≥ 93%, while ERIC-PCR analysis of 5 strains of S. Kentucky revealed 4 ERIC types, clustered in 2 main groups A and B with similarity 75%. We studied the effect of silver nanoparticles (Ag-NPs) on 10 antibiotic resistant strains of S. Enteritidis and S. Kentucky. The broth microdilution minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were detected. Evaluation of the affection using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed different ratios of Ag-NPs and microorganism as well as at different contact time ended finally with morphological alteration of the bacteria. We submitted new method in vivo to explore the activity of nanosilver in chicken. KEY POINTS: • Importance of ERIC-PCR to determine the relatedness between Salmonella isolates. • Effect of silver nanoparticles to confront the antibacterial resistance. • Studying the effect of silver nanoparticles in vivo on infected chicken with Salmonella.
从埃及北部西奈半岛五个大城市的食品样本、腹泻犊牛的粪便、家禽和医院患者中获得了 65 株从埃及亚历山大大学兽医学院微生物系获得的肠炎沙门氏菌分离株。65 株肠炎沙门氏菌分离株均含有 invA 基因，分为 11 种肠炎沙门氏菌血清型，以肠炎沙门氏菌和肯塔基州沙门氏菌血清型为主。采用 18 种不同类别的抗生素对其耐药模式进行了特征描述。约 80%的分离株为多重耐药（MDR）。对 7 株肠炎沙门氏菌进行肠杆菌重复基因间 PCR（ERIC-PCR）分型显示，有 5 个聚类，相似度为 25%。肠炎沙门氏菌聚类为 2 个主要组 A 和 B。组 A 有 2 个人源菌株（HE2 和 HE3）和 1 个食品来源菌株（FE7），相似度为 99%。B 组分为 B1（FE2）和 B2（FE3），相似度比≥93%，而对 5 株肯塔基州沙门氏菌的 ERIC-PCR 分析显示，有 4 种 ERIC 型，聚类为 2 个主要组 A 和 B，相似度为 75%。我们研究了银纳米粒子（Ag-NPs）对 10 株耐药性肠炎沙门氏菌和肯塔基州沙门氏菌的影响。检测了肉汤微量稀释法最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。通过扫描电子显微镜（SEM）和透射电子显微镜（TEM）评价其影响，结果显示不同比例的 Ag-NPs 与微生物以及不同的接触时间最终导致细菌形态发生变化。我们提出了一种新的体内方法来探索纳米银在鸡体内的活性。要点：•ERIC-PCR 对确定沙门氏菌分离株之间的亲缘关系很重要。•银纳米粒子对抗菌耐药性的影响。•研究银纳米粒子在感染沙门氏菌的鸡体内的体内作用。
