• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

禽流感病毒 A H9N2 的 PB2 蛋白的氨基酸残基 253 和 591 有助于哺乳动物发病机制。

Amino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contribute to mammalian pathogenesis.

机构信息

Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong Special Administrative Region, People's Republic of China.

出版信息

J Virol. 2011 Sep;85(18):9641-5. doi: 10.1128/JVI.00702-11. Epub 2011 Jul 6.

DOI:10.1128/JVI.00702-11
PMID:21734052
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3165745/
Abstract

We investigated the tropism, host responses, and virulence of two variants of A/Quail/Hong Kong/G1/1997 (H9N2) (H9N2/G1) with D253N and Q591K in the PB2 protein in primary human macrophages and bronchial epithelium in vitro and in mice in vivo. Virus with PB2 D253N and Q591K had greater polymerase activity in minireplicon assays, induced more tumor necrosis factor alpha (TNF-α) in human macrophages, replicated better in differentiated normal human bronchial epithelial (NHBE) cells, and was more pathogenic for mice. Taken together, our studies help define the viral genetic determinants that contribute to pathogenicity of H9N2 viruses.

摘要

我们研究了两种 A/Quail/Hong Kong/G1/1997（H9N2）（H9N2/G1）变体在原代人巨噬细胞和体外支气管上皮细胞以及体内小鼠中的嗜性、宿主反应和毒力，这两种变体在 PB2 蛋白中有 D253N 和 Q591K 突变。具有 PB2 D253N 和 Q591K 的病毒在 minireplicon 测定中具有更高的聚合酶活性，在人巨噬细胞中诱导更多的肿瘤坏死因子α（TNF-α），在分化正常的人支气管上皮（NHBE）细胞中复制更好，对小鼠的致病性更强。综上所述，我们的研究有助于确定导致 H9N2 病毒致病性的病毒遗传决定因素。

相似文献

1
Amino acid residues 253 and 591 of the PB2 protein of avian influenza virus A H9N2 contribute to mammalian pathogenesis.禽流感病毒 A H9N2 的 PB2 蛋白的氨基酸残基 253 和 591 有助于哺乳动物发病机制。
J Virol. 2011 Sep;85(18):9641-5. doi: 10.1128/JVI.00702-11. Epub 2011 Jul 6.
2
Assessment of the internal genes of influenza A (H7N9) virus contributing to high pathogenicity in mice.评估导致小鼠高致病性的流感 A（H7N9）病毒的内部基因。
J Virol. 2015 Jan;89(1):2-13. doi: 10.1128/JVI.02390-14. Epub 2014 Oct 15.
3
Virulence determinants in the PB2 gene of a mouse-adapted H9N2 virus.鼠源适应 H9N2 病毒 PB2 基因的毒力决定因子。
J Virol. 2015 Jan;89(1):877-82. doi: 10.1128/JVI.01775-14. Epub 2014 Oct 22.
4
Double mutations in the H9N2 avian influenza virus PB2 gene act cooperatively to increase viral host adaptation and replication for human infections.H9N2 禽流感病毒 PB2 基因中的双重突变协同作用，增加了病毒对人类感染的宿主适应性和复制能力。
J Gen Virol. 2021 Jun;102(6). doi: 10.1099/jgv.0.001612.
5
Prevailing PA Mutation K356R in Avian Influenza H9N2 Virus Increases Mammalian Replication and Pathogenicity.禽流感H9N2病毒中普遍存在的PA突变K356R增加了在哺乳动物中的复制和致病性。
J Virol. 2016 Aug 26;90(18):8105-14. doi: 10.1128/JVI.00883-16. Print 2016 Sep 15.
6
The D253N Mutation in the Polymerase Basic 2 Gene in Avian Influenza (H9N2) Virus Contributes to the Pathogenesis of the Virus in Mammalian Hosts.聚合酶基本 2 基因中的 D253N 突变有助于禽流感（H9N2）病毒在哺乳动物宿主中的发病机制。
Virol Sin. 2018 Dec;33(6):531-537. doi: 10.1007/s12250-018-0072-8. Epub 2018 Dec 19.
7
Molecular basis of efficient replication and pathogenicity of H9N2 avian influenza viruses in mice.H9N2 禽流感病毒在小鼠中高效复制和致病的分子基础。
PLoS One. 2012;7(6):e40118. doi: 10.1371/journal.pone.0040118. Epub 2012 Jun 29.
8
Mouse-adapted H9N2 influenza A virus PB2 protein M147L and E627K mutations are critical for high virulence.小鼠适应的 H9N2 流感 A 病毒 PB2 蛋白 M147L 和 E627K 突变对于高致病性至关重要。
PLoS One. 2012;7(7):e40752. doi: 10.1371/journal.pone.0040752. Epub 2012 Jul 10.
9
PA from a Recent H9N2 (G1-Like) Avian Influenza a Virus (AIV) Strain Carrying Lysine 367 Confers Altered Replication Efficiency and Pathogenicity to Contemporaneous H5N1 in Mammalian Systems.来自携带赖氨酸 367 的最近 H9N2（G1 样）禽流感病毒（AIV）株的 PA 赋予哺乳动物系统中同时代的 H5N1 改变的复制效率和致病性。
Viruses. 2020 Sep 20;12(9):1046. doi: 10.3390/v12091046.
10
Mouse-adapted H9N2 avian influenza virus causes systemic infection in mice.鼠适应 H9N2 禽流感病毒可引起小鼠全身性感染。
Virol J. 2019 Nov 12;16(1):135. doi: 10.1186/s12985-019-1227-4.

引用本文的文献

1
Converging Transmission Routes of the Highly Pathogenic Avian Influenza H5N1 Clade 2.3.4.4b Virus in Uruguay: Phylogeographic Insights into Its Spread Across South America.乌拉圭高致病性禽流感H5N1 2.3.4.4b分支病毒的汇聚传播途径：对其在南美洲传播的系统地理学见解
Pathogens. 2025 Aug 8;14(8):793. doi: 10.3390/pathogens14080793.
2
Evaluation of pandemic potential of the genotype 4 (G4) swine influenza virus using and cultures of the human respiratory tract.利用人类呼吸道的原代和传代培养物评估4型（G4）猪流感病毒的大流行潜力。
J Gen Virol. 2025 Jul;106(7). doi: 10.1099/jgv.0.002133.
3
Viral characterization of the reassortants between canine influenza H3N2 and human pandemic (2009) H1N1 and avian H9N2 viruses in canine ex vivo tracheal explants.犬类离体气管外植体中犬流感H3N2与人类大流行（2009年）H1N1以及禽H9N2病毒重组体的病毒特性分析
Virol J. 2025 Jul 1;22(1):218. doi: 10.1186/s12985-025-02836-1.
4
Genetic diversity of H9N2 avian influenza viruses in poultry across China and implications for zoonotic transmission.中国家禽中H9N2禽流感病毒的遗传多样性及其对人畜共患病传播的影响。
Nat Microbiol. 2025 Jun;10(6):1378-1392. doi: 10.1038/s41564-025-02002-x. Epub 2025 Jun 3.
5
Cross-species and mammal-to-mammal transmission of clade 2.3.4.4b highly pathogenic avian influenza A/H5N1 with PB2 adaptations.具有PB2适应性的2.3.4.4b分支高致病性甲型H5N1禽流感病毒的跨物种及哺乳动物间传播
Nat Commun. 2025 Mar 6;16(1):2232. doi: 10.1038/s41467-025-57338-z.
6
Origin, pathogenicity, and transmissibility of a human isolated influenza A(H10N3) virus from China.一株源自中国的人源甲型流感病毒A(H10N3)的起源、致病性及传播性
Emerg Microbes Infect. 2025 Dec;14(1):2432364. doi: 10.1080/22221751.2024.2432364. Epub 2024 Dec 9.
7
Comparative analysis of PB2 residue 627E/K/V in H5 subtypes of avian influenza viruses isolated from birds and mammals.从鸟类和哺乳动物中分离出的H5亚型禽流感病毒PB2基因627位氨基酸E/K/V残基的比较分析
Front Vet Sci. 2023 Sep 1;10:1250952. doi: 10.3389/fvets.2023.1250952. eCollection 2023.
8
Continued Circulation of Highly Pathogenic H5 Influenza Viruses in Vietnamese Live Bird Markets in 2018-2021.2018-2021 年越南活禽市场中高致病性 H5 流感病毒的持续循环。
Viruses. 2023 Jul 21;15(7):1596. doi: 10.3390/v15071596.
9
An intermediate state allows influenza polymerase to switch smoothly between transcription and replication cycles.中间状态允许流感聚合酶在转录和复制循环之间顺畅切换。
Nat Struct Mol Biol. 2023 Aug;30(8):1183-1192. doi: 10.1038/s41594-023-01043-2. Epub 2023 Jul 24.
10
The synergistic effect of residues 32T and 550L in the PA protein of H5 subtype avian influenza virus contributes to viral pathogenicity in mice.H5 亚型禽流感病毒 PA 蛋白中残基 32T 和 550L 的协同作用有助于病毒在小鼠中的致病性。
PLoS Pathog. 2023 Jul 3;19(7):e1011489. doi: 10.1371/journal.ppat.1011489. eCollection 2023 Jul.

本文引用的文献

1
Biological and structural characterization of a host-adapting amino acid in influenza virus.流感病毒宿主适应性氨基酸的生物学和结构特征。
PLoS Pathog. 2010 Aug 5;6(8):e1001034. doi: 10.1371/journal.ppat.1001034.
2
Evidence for avian and human host cell factors that affect the activity of influenza virus polymerase.流感病毒聚合酶活性的宿主细胞因子的研究进展：禽类和人类宿主细胞因子的影响。
J Virol. 2010 Oct;84(19):9978-86. doi: 10.1128/JVI.01134-10. Epub 2010 Jul 14.
3
PB2 residue 271 plays a key role in enhanced polymerase activity of influenza A viruses in mammalian host cells.PB2 残基 271 在流感病毒在哺乳动物宿主细胞中增强聚合酶活性方面发挥关键作用。
J Virol. 2010 May;84(9):4395-406. doi: 10.1128/JVI.02642-09. Epub 2010 Feb 24.
4
Influenza H5N1 and H1N1 virus replication and innate immune responses in bronchial epithelial cells are influenced by the state of differentiation.流感 H5N1 和 H1N1 病毒在支气管上皮细胞中的复制和固有免疫反应受分化状态的影响。
PLoS One. 2010 Jan 15;5(1):e8713. doi: 10.1371/journal.pone.0008713.
5
Adaptive strategies of the influenza virus polymerase for replication in humans.流感病毒聚合酶在人体中复制的适应策略。
Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21312-6. doi: 10.1073/pnas.0911915106. Epub 2009 Dec 7.
6
Viral genetic determinants of H5N1 influenza viruses that contribute to cytokine dysregulation.导致细胞因子失调的H5N1流感病毒的病毒遗传决定因素。
J Infect Dis. 2009 Oct 1;200(7):1104-1112. doi: 10.1086/605606.
7
Nuclear factor 90 negatively regulates influenza virus replication by interacting with viral nucleoprotein.核因子90通过与病毒核蛋白相互作用负向调节流感病毒复制。
J Virol. 2009 Aug;83(16):7850-61. doi: 10.1128/JVI.00735-09. Epub 2009 Jun 3.
8
Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice.多种氨基酸替换参与了H9N2禽流感病毒对小鼠的适应性。
Vet Microbiol. 2009 Jul 2;138(1-2):85-91. doi: 10.1016/j.vetmic.2009.03.010. Epub 2009 Mar 13.
9
Selection of H5N1 influenza virus PB2 during replication in humans.人感染H5N1流感病毒复制过程中PB2基因的选择
J Virol. 2009 May;83(10):5278-81. doi: 10.1128/JVI.00063-09. Epub 2009 Mar 4.
10
Host determinant residue lysine 627 lies on the surface of a discrete, folded domain of influenza virus polymerase PB2 subunit.宿主决定簇残基赖氨酸627位于流感病毒聚合酶PB2亚基一个离散折叠结构域的表面。
PLoS Pathog. 2008 Aug 29;4(8):e1000136. doi: 10.1371/journal.ppat.1000136.