College of Life Science, Henan Normal University, Xinxiang, Henan Province, China.
Genet Mol Biol. 2011 Apr;34(2):304-9. doi: 10.1590/S1415-4757201100500001. Epub 2011 Apr 1.
Rapidly proliferating tissue may require enhanced DNA repair capacity in order to avoid fixation of promutagenic DNA lesions to mutations. Partial hepatectomy (PH) triggers cell proliferation during liver regeneration (LR). However, little is known on how DNA repair genes change and how they are regulated at the transcriptional level during LR. In the present study, the Rat Genome 230 2.0 array was used to detect the expression profiles of DNA repair genes during LR, and differential expression of selected genes was confirmed by real-time RT-PCR. 69 DNA repair genes were found to be associated with LR, more than half of which distributed in a cluster characterized by a gradual increase at 24-72h and then returning to normal. The expression of base excision repair- and transcription-coupled repair-related genes was enhanced in the early and intermediate phases of LR, whereas the expression of genes related to HR, NHEJ and DNA cross-link repair, as well as DNA polymerases and related accessory factors, and editing or processing nucleases, were mainly enhanced in the intermediate phase. The expression changes of genes in DNA damage response were complicated throughout the whole LR. Our data also suggest that the expression of most DNA repair genes may be regulated by the cell cycle during LR.
快速增殖的组织可能需要增强 DNA 修复能力，以避免将诱变 DNA 损伤固定为突变。部分肝切除术（PH）在肝再生（LR）期间触发细胞增殖。然而，对于 DNA 修复基因在 LR 期间如何改变以及它们在转录水平上如何被调控，人们知之甚少。在本研究中，使用 Rat Genome 230 2.0 阵列来检测 LR 过程中 DNA 修复基因的表达谱，并通过实时 RT-PCR 确认选定基因的差异表达。发现 69 个 DNA 修复基因与 LR 相关，其中一半以上分布在一个特征为 24-72 小时逐渐增加然后恢复正常的簇中。碱基切除修复和转录偶联修复相关基因的表达在 LR 的早期和中期增强，而与 HR、NHEJ 和 DNA 交联修复以及 DNA 聚合酶和相关辅助因子以及编辑或加工核酸酶相关的基因的表达主要在中期增强。整个 LR 过程中，DNA 损伤反应中基因的表达变化很复杂。我们的数据还表明，大多数 DNA 修复基因的表达可能在 LR 过程中受到细胞周期的调控。
