Charlton B R, Barr B C, Castro A E, Davis P L, Reynolds B J
California Veterinary Diagnostic Laboratory System, School of Veterinary Medicine, University of California-Davis, Turlock 95381.
Avian Dis. 1990 Jul-Sep;34(3):787-90.
Herpesvirus infection was diagnosed in a toucan. The herpesvirus was isolated from the liver and identified by electron microscopy in the liver and in cell culture. A negative immunofluorescent reaction was obtained when virus-infected cell cultures were reacted with a conjugate to the herpesvirus of Pacheco's disease. The main pathologic finding in the toucan consisted of a severe necrotizing hepatitis with intranuclear inclusions in the liver and spleen. A presumptive diagnosis of chlamydiosis was also made, based on a positive direct fluorescent monoclonal antibody reaction to chlamydial antigens in impression smears of liver and spleen. Chlamydial isolation attempts were unsuccessful. The toucan had been in contact with two macaws that had died 5 days before the toucan died and were diagnosed by histology as having herpesvirus hepatitis.
在一只巨嘴鸟身上诊断出疱疹病毒感染。从肝脏中分离出疱疹病毒，并通过电子显微镜在肝脏和细胞培养物中进行鉴定。当病毒感染的细胞培养物与针对帕切科病疱疹病毒的结合物反应时，获得了阴性免疫荧光反应。这只巨嘴鸟的主要病理发现包括严重的坏死性肝炎，肝脏和脾脏中有核内包涵体。基于对肝脏和脾脏印片上衣原体抗原的阳性直接荧光单克隆抗体反应，也做出了衣原体病的初步诊断。衣原体分离尝试未成功。这只巨嘴鸟曾与两只金刚鹦鹉接触，这两只金刚鹦鹉在巨嘴鸟死亡前5天死亡，经组织学诊断为疱疹病毒性肝炎。
